30 × 104 to 1.23 × 104 copies/μL. The healthy bladder mucosa samples were negative for both bovine papillomaviruses. Once the results were confirmed by conventional PCR and direct sequencing, the quantitative PCR assay developed in this study was shown to be a sensitive and specific tool for detecting and quantifying the E6 ORF of bovine papillomavirus 2 and 13 in a variety of clinical samples. Our findings of identification of bovine papillomavirus 2 and 13 DNA in urothelial tumors from cattle suffering from bovine enzootic hematuria agree with data from previous studies, representing the first detection of bovine papillomavirus 13 DNA in malignant bladder lesions of cattle from Brazil.Triple pelvic osteotomy (TPO) is a prophylactic surgical procedure performed on dogs with canine hip dysplasia. The procedure is indicated in skeletally immature dogs without secondary osteoarthritis (OA). It has been suggested that 60° of rotation is excessive and is associated with poor outcome. The objective of the study was to assess the medium term outcome in dogs having undergone triple pelvic osteotomy (TPO) using 60° dedicated plates. Nine TPOs were performed in seven dogs with hip dysplasia. Eight of nine hips had 72-100% osseous union at the time of revisit. The mean time to final radiographic recheck was 200 days (range, 185-229 days). The mean time to follow-up was 11.5 months (range 11-12 months). All 7 dogs had regained full function and did not require supplemental analgesia. Pelvic canal narrowing was noted in the two dogs with bilateral surgeries, but no clinical consequences were noted according to owner's statement.If more than 40 degrees reduction angles at Ortolani test, 60° of rotation of the acetabulum can be used successfully in dogs with hip dysplasia. At the time of mid-term follow-up, all dogs in this case series had full function.High elimination rates and the concern for horse welfare are important issues in endurance riding. An improved understanding of the causes of elimination could increase completion rates in this sport. We have identified pre-ride risk factors that allow an assessment of potential elimination before the ride. A longitudinal cohort study was performed among 49 healthy horses competing in the 160 km endurance ride at the 2016 World Championship of Endurance Riding in Samorin/Slovakia. Blood samples were drawn before the ride. For statistical evaluation, horses were categorized in three groups finishers, lame and metabolically eliminated horses. Risk factors were calculated for each group using multinomial logistic regression. A 1% increase in hematocrit levels was associated with a higher OR for elimination (lameness OR 1.26, p = 0.017; metabolic OR 1.34, p = 0.010). Furthermore, increased potassium values correlated negatively with the race outcome. For a 1 mmol/l increase in potassium, the lameness OR was 4.21, p = 0.039 and metabolic OR was 1.15, p = 0.848. Eight-year-old horses had a 100% elimination rate and survival analyses showed a significantly higher hazard for elimination (p = 0.025). We thus conclude that age and hydration affect the outcome of elite endurance rides. Further investigation of age as a risk factor seems to be clinically relevant and adjustments of FEI qualification modes may be appropriate.The bovine viral diarrhea virus (BVDV) consists of two species and various subspecies of closely related viruses of varying antigenicity, cytopathology, and virulence-induced pathogenesis. Despite the great ongoing efforts to control and prevent BVDV outbreaks and the emergence of new variants, outbreaks still reported throughout the world. In this review, we are focusing on the molecular biology of BVDV, its molecular pathogenesis, and the immune response of the host against the viral infection. Special attention was paid to discuss some immune evasion strategies adopted by the BVDV to hijack the host immune system to ensure the success of virus replication. Vaccination is one of the main strategies for prophylaxis and contributes to the control and eradication of many viral diseases including BVDV. https://www.selleckchem.com/products/pmx-53.html We discussed the recent advances of various types of currently available classical and modern BVDV vaccines. However, with the emergence of new strains and variants of the virus, it is urgent to find some other novel targets for BVDV vaccines that may overcome the drawbacks of some of the currently used vaccines. Effective vaccination strategy mainly based on the preparation of vaccines from the homologous circulating strains. The BVDV-E2 protein plays important role in viral infection and pathogenesis. We mapped some important potential neutralizing epitopes among some BVDV genomes especially the E2 protein. These novel epitopes could be promising targets against the currently circulating strains of BVDV. More research is needed to further explore the actual roles of these epitopes as novel targets for the development of novel vaccines against BVDV. These potential vaccines may contribute to the global eradication campaign of the BVDV.Ulnar trochlear notch (UTN) subchondral bone sclerosis is observed in elbow dysplasia (ED) associated with the medial coronoid disease. However, its evaluation is based on a simple visual examiner assessment of bone radio-opacity level and is considered subjective. The purpose of this study was to objectively characterize the radiographic opacity of the ulnar trochlear notch (UTN) subchondral bone in mediolateral elbow projections classified, using the International Elbow Working Group guidelines. Records and mediolateral flexed elbow images from the Danish Kennel Club database for the ED screening scheme between 2012 and 2018 were available. Of the dogs in the database, those with an ED-negative status in the left limb were identified. From these, 20 dogs each having a status free from ED, or with Grade 1, 2, or 3 in the right limb, were randomly chosen. Joints with primary ununited anconeal process were excluded from the sample. A template was developed using the ImageJ software, for computer UTN sclerosis ed by pixel intensity) though statistically significant in dysplastic joints compared with contralateral normal joints are slight and are thus problematic for computer-aided assessments of UTN sclerosis.

Obesity and obesity-related disorders, such as type 2 diabetes have been progressively increasing worldwide and treatments have failed to counteract their progression. Growing evidence have demonstrated that gut microbiota is associated with the incidence of these pathologies. Hence, the identification of new nutritional compounds, able to improve health through a modulation of gut microbiota, is gaining interest. In this context, the aim of this study was to investigate the gut-driving effects of rhubarb extract in a context of diet-induced obesity and diabetes. Eight weeks old C57BL6/J male mice were fed a control diet (CTRL), a high fat and high sucrose diet (HFHS) or a HFHS diet supplemented with 0.3% (g/g) of rhubarb extract for eight weeks. Rhubarb supplementation fully prevented HFHS-induced obesity, diabetes, visceral adiposity, adipose tissue inflammation and liver triglyceride accumulation, without any modification in food intake. By combining sequencing and qPCR methods, we found that all these effects were associated with a blooming of Akkermansia muciniphila, which is strongly correlated with increased expression of Reg3γ in the colon. Our data showed that rhubarb supplementation is sufficient to protect against metabolic disorders induced by a diet rich in lipid and carbohydrates in association with a reciprocal interaction between Akkermansia muciniphila and Reg3γ.Improvement of the medical textile industry has received more attention recently, especially with widespread of microbial and viral infections. Medical textiles with new properties, such as bacterial pathogens self-cleaning, have been explored with nanotechnology. In this study, an endophytic actinomycetes strain of Streptomyces laurentii R-1 was isolated from the roots of the medicinal plant Achillea fragrantissima. This is used as a catalyst for the mediated biosynthesis of silver nanoparticles (Ag-NPs) for applications in the textile industry. The biosynthesized Ag-NPs were characterized using UV-vis spectroscopy, Fourier transform infrared (FT-IR), transmission electron microscopy (TEM), and X-ray Diffraction (XRD), which confirmed the successful formation of crystalline, spherical metal nanoparticles. The biosynthesized Ag-NPs exhibited broad-spectrum antibacterial activity. Our data elucidated that the biosynthesized Ag-NPs had a highly cytotoxic effect against the cancerous caco-2 cell line. The selected safe dose of Ag-NPs for loading on cotton fabrics was 100 ppm, regarding their antibacterial activity and safe cytotoxic efficacy. Interestingly, scanning electron microscope connected with energy dispersive X-ray spectroscopy (SEM-EDX) of loaded cotton fabrics demonstrated the smooth distribution of Ag-NPs on treated fabrics. https://www.selleckchem.com/products/pfk158.html The obtained results highlighted the broad-spectrum activity of nano-finished fabrics against pathogenic bacteria, even after 5 and 10 washing cycles. This study contributes a suitable guide for the performance of green synthesized NPs for utilization in different biotechnological sectors.Background Data on Roma women's experience of violence from their male partners are very scarce. We explored the process of actual domestic violence against Roma women, the threat of violence and its health consequences. We further focused on barriers in the availability of specialized support services aimed at eliminating domestic violence. Methods The sample included 20 Roma women living throughout Slovakia scattered among the majority (45.0%), in crisis centers and sheltered houses (40.0%), and in segregated Roma settlements (15.0%). Data were obtained through qualitative research by means of semi-structured interviews in 20 individual case studies. All 20 women had experienced a combination of violence physical, psychological and economic, all of them connected with social isolation. Results Prevailing gender stereotypes are a precondition of domestic violence against women, regardless of their status. Violence against Roma women resulted in several health consequences, and all of the 20 women suffered from these. Most of them reported general psychological problems (75%), among which anxiety and depression (25%), headache (25%), weight loss (10%) and health problems connected with motor activity (5%). The barriers include lack of awareness among Roma women of any specialised support services and the absence of such services for abused women in the region. Conclusions Domestic violence results in serious psychological and physical health consequences. Violence elimination is generally set up without a specific ethnic or gender approach. Disregard of these specifics can lead to deepening of the uneven position of Roma women within the family, community and society, and the acceptance of violence against Roma women.In our previous study, Hwang-Ryun-Hae-Dok-Tang, which contains berberine (BBR) as a main active ingredient, inhibited cytochrome P450 (CYP) 2D6 in a quasi-irreversible manner. However, no information is available on the detailed mechanism of BBR-induced CYP2D6 inhibition. Thus, the present study aimed to characterize the inhibition mode and kinetics of BBR and its analogues against CYP2D6 using pooled human liver microsomes (HLM). BBR exhibited selective quasi-irreversible inhibition of CYP2D6 with inactivation rate constant (kinact) of 0.025 min-1, inhibition constant (KI) of 4.29 µM, and kinact/KI of 5.83 mL/min/µmol. In pooled HLM, BBR was metabolized to thalifendine (TFD), demethyleneberberine (DMB), M1 (proposed as demethylene-TFD), and to a lesser extent berberrubine (BRB), showing moderate metabolic stability with a half-life of 35.4 min and a microsomal intrinsic clearance of 7.82 µL/min/mg protein. However, unlike BBR, those metabolites (i.e., TFD, DMB, and BRB) were neither selective nor potent inhibitors of CYP2D6, based on comparison of half-maximal inhibitory concentration (IC50). Notably, TFD, but not DMB, exhibited metabolism-dependent CYP2D6 inhibition as in the case of BBR, which suggests that methylenedioxybenzene moiety of BBR may play a critical role in the quasi-irreversible inhibition. Moreover, the metabolic clearance of nebivolol (β-blocker; CYP2D6 substrate) was reduced in the presence of BBR. The present results warrant further evaluation of BBR-drug interactions in clinical situations.

Following the development of the tramadol crisis currently affecting countries in the Middle East, and Africa, there has been increasing international interest in the regulation of tramadol. This study investigates the misuse of tramadol in patients presenting to emergency departments across Europe. Data from 32 emergency departments in 21 countries were extracted from the Euro-DEN Plus database for the 4-year period from 1st January 2014 to 31st December 2017. Of the reported 24,957 emergency department presentations, tramadol misuse was reported in 105 (0.4% presentations). Tramadol misuse was most common in Bratislava (Slovakia) (n=11, 7.5% of all presentations to this centre), Riga (Latvia) (n=4, 4.9%) and Munich (Germany) (n=17, 2.9%). On arrival, 14 (13.3%) of presentations were in coma/GCS≤8 and nine of these had a respiratory rate less then 12 per minute. These presentations potentially pose a significant burden on EDs with a large proportion requiring admission to hospital for ongoing care.On March 11th, 2020, the WHO declared the SARS-Cov-2 pandemic. Syndromes have been detected in relation to COVID-19 such as encephalitis, acute necrotizing hemorrhagic encephalopathy and cerebrovascular complications. There are also cases of peripheral nervous system involvement. Our case would be the 3rd patient with MFS associated with COVID-19 as far as we know. We present a 51 years old female diagnosed with MFS two weeks after COVID-19. RT-PCR to SARS-CoV-2 was negative but IgG was positive. Most of the cases were mild or moderate with typical signs and symptoms. All were treated with IV immunoglobulin with good response in most cases. https://www.selleckchem.com/products/pds-0330.html Despite the short evolution time of the cases surviving the current pandemic, the description of cases of post-infectious neurological syndromes suggests that this is probably not an infrequent complication in the subacute stage of Covid-19 disease.Objective To determine the association between oil pollution and miscarriage, stillbirth, and infant death in the Niger Delta region of Nigeria. Methods A retrospective cohort study was undertaken of pregnant women (aged 18-45 years) who attended selected health facilities in regions with high and low exposure to oil pollution from May 14, 2018, to September 27, 2018. A multistage sampling technique was used to randomly select a representative of women with high and low exposure to oil pollution. An interviewer-administered questionnaire was used for data collection. Bivariate and multivariable logistic regression analyses were employed to adjust for confounding factors of miscarriage, stillbirth, and infant death. Results In total, 1564 pregnant women were included in the study. Women with high exposure to oil pollution were more likely to experience stillbirth (odds ratio [OR] 1.806; 95% confidence interval [CI] 1.177-2.770) and infant death (OR 2.162; 95% CI 1.409-3.317). However, after adjusting for potential confounders, only infant death was associated with high exposure (adjusted OR 1.843; 95% CI 1.146-2.962). No association was found between miscarriage and high exposure to oil pollution. Conclusion Women with high exposure to oil pollution are at higher risk of infant death.COVID‐19 infection can cause a severe pneumonia which, in some cases, can lead to admission in intensive care unit for respiratory support.1 In severe cases, systemic thrombotic complication has been described, including cerebrovascular disease (5.7‐23% of cases).2,3The Lake Huron ecosystem is unique among the Laurentian Great Lakes in that its surface area encompasses three distinct basins. This ecosystem recently experienced significant ecological restructuring characterized by changes in primary production, species dominance and abundances, and top predator energy dynamics. However, much of the evidence for this restructuring has been largely derived from biomonitoring data derived from long term sampling of the lake's Main Basin. Here, we examined polychlorinated biphenyl (PCB) concentrations and the stable isotopes of carbon (δ13 C) and nitrogen (δ15 N) in rainbow smelt (Osmerus mordax), bloater (Coregonus hoyi) and round goby (Neogobius melanostomus) to determine spatial variability in these environmental markers as indicators of the ubiquity of trophic restructuring throughout Lake Huron. Stable isotopes indicated North Channel fish occupied trophic positions between 0.5 - 1.0 lower relative to Main Basin and Georgian Bay conspecifics, respectively. Sum PCB concentrations for 41 congeners were highest for fish from the Main Basin (27.5 ± 3.0 ng·g-1 wet wt) and Georgian Bay (26.3 ± 3.4 ng·g-1 ) relative to North Channel (13.6 ± 1.2 ng·g-1 ) fish. Discriminant functions analysis demonstrated basin-specific PCB congener profiles with individual species also having distinct profiles dependent on their basin of collection. These bioaccumulation patterns among Lake Huron forage fish mirror those reported for lake trout in this lake and indicate that the degree of food-web ecological restructuring in Lake Huron is not equivalent across the basins. Specifically, basin-specific PCB congener profiles demonstrated among Lake Huron secondary and top predator consumer species are likely dictated by cross-basin differences in zooplankton community ecology and trophodynamics that can regulate the efficiencies of prey energy transfer and PCB congener bioaccumulation patterns in aquatic food-webs. This article is protected by copyright. All rights reserved.The validity of soil toxicity databases for predicting ecological impacts in the field is rarely explored. This work was set up to test if laboratory toxicity data and the combined concepts of metal availability and mixture toxicity can predict ecological impact in mining-affected soils. Metal and arsenic (As) contamination gradients were sampled around five different mines in Mexico where plant cover and abundances exhibited clear dose-related responses. Soils were analyzed for total and isotopically exchangeable (labile) concentrations of Ni, Cu, Cd, Pb, and As and for soil properties affecting the availability of these elements. Six different indices of toxic doses were compared to evaluate their accuracy in describing the field response expressed as relative abundance and cover. Each index was based on a different method to calculate the sum of toxic units (ΣTU) in soil, with 1 TU equal to the concentration of the element in soil yielding 50% adverse effect on plants with median sensitivity as recorded in a recent database of salt spiked soils.

Applications in Plant Sciences is published by Wiley Periodicals, Inc. on behalf of the Botanical Society of America.Premise A novel set of microsatellite markers was developed for Juglans sigillata (Juglandaceae), an endemic walnut species in southwestern China, to facilitate cultivar identification and future investigations into the genetic structure and domestication history of this species and its close relatives. Methods and Results We developed 32 microsatellite loci for J. sigillata using genomic data and used them to examine 60 individuals from three natural populations. A high level of polymorphism was detected by these primers, with up to eight alleles observed per locus, and an average of four alleles across populations. The levels of observed and expected heterozygosity ranged from 0.000-1.000 and 0.000-0.785, respectively. All but two of the loci were also successfully amplified in three closely related Eurasian Juglans species (J. regia, J. cathayensis, and J. mandshurica). Conclusions The microsatellite loci identified here provide a powerful resource for examining the genetic structure and domestication history of Juglans, as well as identification of its cultivars. © 2020 Xu et al. Applications in Plant Sciences is published by Wiley Periodicals, Inc. on behalf of the Botanical Society of America.Premise Polymorphic microsatellite markers were developed to study genetic diversity and genetic structure of populations of the locally endangered species Tofieldia calyculata (Tofieldiaceae). Methods and Results Nineteen polymorphic microsatellite loci were developed using DNA-enriched libraries sequenced by Ilumina technology and were then used to genotype 101 individuals from five populations from Austria, Slovakia, Poland, and the Czech Republic. Of the markers tested, 68% were polymorphic in four of the five investigated populations, with one marker polymorphic in all populations. The number of alleles per locus in the populations ranged from one to 11. Levels of observed and expected heterozygosity ranged from 0.00 to 0.75 and from 0.00 to 0.84, respectively. Moreover, six of the 19 loci amplified when tested in the congeneric species T. pusilla. Conclusions The 19 newly developed microsatellite markers can be used to describe genetic diversity and population structure of populations of T. calyculata. © 2020 Vlasta et al. Applications in Plant Sciences is published by Wiley Periodicals, Inc. on behalf of the Botanical Society of America.An emerging approach to ecosystem monitoring involves the use of physiological biomarker analyses in combination with gene transcription assays. For the first time, we employed these tools to evaluate the Pacific razor clam (Siliqua patula), which is important both economically and ecologically, as a bioindicator species in the northeast Pacific. Our objectives were to (1) develop biomarker and gene transcription assays with which to monitor the health of the Pacific razor clam, (2) acquire baseline biomarker and gene transcription reference ranges for razor clams, (3) assess the relationship between physiological and gene transcription assays and (4) determine if site-level differences were present. Pacific razor clams were collected in July 2015 and 2016 at three sites within each of two national parks in southcentral Alaska. In addition to determining reference ranges, we found differences in biomarker assay and gene transcription results between parks and sites which indicate variation in both large-scale and local environmental conditions. Our intent is to employ these methods to evaluate Pacific razor clams as a bioindicator of nearshore ecosystem health. Links between the results of the biomarker and gene transcription assays were observed that support the applicability of both assays in ecosystem monitoring. However, we recognize the need for controlled studies to examine the range of responses in physiology and gene transcripts to different stressors. © 2020 Bowen et al.Insulin-stimulated glucose transport is a characteristic property of adipocytes and muscle cells and involves the regulated delivery of glucose transporter (GLUT4)-containing vesicles from intracellular stores to the cell surface. Fusion of these vesicles results in increased numbers of GLUT4 molecules at the cell surface. In an attempt to overcome some of the limitations associated with both primary and cultured adipocytes, we expressed an epitope- and GFP-tagged version of GLUT4 (HA-GLUT4-GFP) in HeLa cells. Here we report the characterisation of this system compared to 3T3-L1 adipocytes. We show that insulin promotes translocation of HA-GLUT4-GFP to the surface of both cell types with similar kinetics using orthologous trafficking machinery. While the magnitude of the insulin-stimulated translocation of GLUT4 is smaller than mouse 3T3-L1 adipocytes, HeLa cells offer a useful, experimentally tractable, human model system. Here, we exemplify their utility through a small-scale siRNA screen to identify GOSR1 and YKT6 as potential novel regulators of GLUT4 trafficking in human cells. © 2020 Morris et al.Aims To identify the common and specific molecular mechanisms of three well-defined subtypes of endometriosis (EMs) ovarian endometriosis (OE), peritoneal endometriosis (PE), and deep infiltrating endometriosis (DIE). Methods Four microarray datasets GSE7305 and GSE7307 for OE, E-MTAB-694 for PE, and GSE25628 for DIE were downloaded from public databases and conducted to compare ectopic lesions (EC) with eutopic endometrium (EU) from EMs patients. Differentially expressed genes (DEGs) identified by limma package were divided into two parts common DEGs among three subtypes and specific DEGs in each subtype, both of which were subsequently performed with the Kyoto Encyclopedia of Genes (KEGG) pathway enrichment analysis. https://www.selleckchem.com/products/abtl-0812.html The protein-protein interaction (PPI) network was constructed by common DEGs and five hub genes were screened out from the PPI network. Besides, these five hub genes together with selected interested pathway-related genes were further validated in an independent OE RNA-sequencing dataset GSE105764.

The aim of the article is to describe the development of the principles of medicine based on the evidence (EBM) based on postulates of Robert Koch, Nobel prize winner, protagonist of the "Golden Age" medical bacteriology, founder of a concept of modern microbiology and infectology. Kochs work led to the discovery of a causal relationship between exposure to a specific pathogen and disease on the example of identifying the cause of anthrax - Bacillus anthracis, a disease whose symptoms vary depending on the mode of transmission (gastrointestinal ingestion, cutaneous form on contact and pulmonary manifestations when inhaled). Tuberculosis caused by Kochs bacillus, Mycobacterium tuberculosis, yet still affecting 1.7 billion people (about 25 % of the world's population), in 95 % of cases in developing countries, where poverty and high prevalence of HIV are part of everyday life. Koch also discovered Vibrio cholerae, the pathogen responsible for seven recorded pandemics, and hitherto sporadic epidemics in recent years. https://www.selleckchem.com/MEK.html The main contribution of the Kochs four postulates formulation was the principle, which helped to reveal the causal relationship between the pathogenic microbe to protrude infectious disease and obtain reliable evidence in improving credibility of diagnosis of infectious diseases. Other stages in the development of EBM were formulated by Bradford Hill in his nine principles, which are valid as well for noncommunicable diseases. The subjects of discussion are limitations and restrictions of present EBM and its essentials and the use in rational preventive, diagnostic and treatment strategies.There are many species of arthropods in the Czech Republic and Slovakia that suck blood in humans and cause unpleasant skin reactions. The extent and type of local reaction depends on the number of previous exposures to the given ectoparasite species, which is also related to possible therapeutic interventions. The review summarizes the classes of arthropods that suck blood in humans in the Czech Republic and Slovakia. The most important families of ticks (Ixodidae) and mosquitoes (Culicidae) pose the risk of transmitting the infection to humans. The individual infectious diseases, especially the possibilities of diagnosis and treatment of stages not requiring hospital care, and the most common myths related to this issue are briefly described.ATP-sensitive potassium channels have been an intensively studied type of protein complexes incorporated in the cell membrane for several decades. Their unique function makes them special, as they create a connection between the metabolic state and membrane voltage of the cell. This position of a bridge involved in many cellular cascades allow them to participate in various processes at often surprising positions in nearly all organ systems of the body, from the pancreas, heart muscle or retina, to the central nervous system. This review summarizes the most important roles of ATP-sensitive potassium channels focusing on their possible clinical use within particular organ systems.Non-alcoholic fatty liver disease (NAFLD) is the most common chronic liver disease nowadays. It is referred to as liver manifestation of metabolic syndrome. Given the growing incidence of diabetes and obesity, we can confidently say that we will be, or even are facing, a NAFLD pandemic in the near future. Despite strenuous efforts, there is no causal therapy and the only effective therapeutic modality is a complex lifestyle change encompasses diet and regime changes leading to a weight loss. However, it is clear from many published papers that putting these recommendations into practice and their long-term compliance with patients is very difficult. What can be offered to patients with NAFLD in clinical practice and how can they be rationally treated?Obesity brings problems both for individual health and economic of the whole society. Unfortunately, in addition to serious approaches to the therapy of obesity, lay offers by non-serious entrepreneurs are still prevalent, often leading to alternating stages of meaningless weight reduction with stages of uncontrolled states and overeating, which is negatively reflected in both physical and mental health. It is within the competence of experts to increase the nutritional literacy of patients. To teach patients to critically evaluate lay information and to put serious information into practical life is an important part of education. It is necessary to correct offers of quick weight loss through strict unbalanced diets or ineffective products but experts often do not have the time and motivation to publish in mass media. For 30 years STOB has tried to fill this gap and has developed programs that have led people to change their eating and movement habits. The aim of the intervention is not only education, but through cognitive-behavioral psychotherapy and mindfulness, STOB leads overweight people to transform theoretical knowledge into practical life. The organization of group courses of healthy losing weight is the main program. STOB has developed a short intervention methodology for physicians and offers a "distant" assistance to overweight people through printed materials and internet programs.COVID-19 pandemics places extreme demands on healthcare workers. Mental health support is supposed to be an indispensable part of complex care for workers for the sake of their adequate professional performance and prevention of psychological disturbances and psychiatric disorders. The most common problems connected with hard work conditions are reviewed and specific techniques to deal with acute stress are presented.Antibody identification is an important diagnostic tool that allows us to confirm a past SARS-CoV-2 infection. While antibody tests in the Czech Republic are yet to receive the attention they deserve, in the USA antibody testing has already become part of the diagnostic algorithms. The aim of this publication is to provide comprehensive information on the topic of antibody testing, the kinetics of antibody production, the types of tests and the issue of virus neutralization tests. Special attention is paid to the correct interpretation of test results and the issues surrounding reinfection.