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48 mins ago


We evaluated 35 lesions across the intraventricular septum (UPA, N = 17 vs BPA, N = 18). No difference in volume, linear dimensions or impedance drop was observed in this area between UPA and BPA. However, BPA required half RF time and showed an increased transmurality trend. We then analyzed 73 lesions from the endocardial side (UPA, N = 35 vs BPA, N = 38) and 50 from the epicardial side (UPA, N = 11 vs BPA N = 39) of the ventricular free walls. Lesion transmurality was markedly improved by BPA (P = .030, odds ratio, 23.73 [4.71,31.96]). Ventricular BPA lesions were significantly deeper on the epicardial side (P  less then  .0001) and endocardial side (P = .015). CONCLUSION Bipolar ablation is more likely to create transmural and epicardial lesions in the ventricle wall. Half the time is needed for the creation of comparably deep and large lesions. © 2020 Wiley Periodicals, Inc.In this study, the synthesis of tetramethoxy resorcinarene podands bearing p-toluene arms connected by -SO3- (1) and -CH2O- (2) linkers is presented. In the solid state, the resorcinarene podand 1 forms an intramolecular self-inclusion complex of the p-toluene group, whereas the resorcinarene podand 2 did not show self-inclusion. https://www.selleckchem.com/products/resatorvid.html The conformation of the flexible resorcinarene podands in solution was investigated by 1D and 2D NMR spectroscopic techniques using variable temperature experiments, as well as, with computational methods including conformational search and subsequent density functional theory (DFT) optimization for representative structures. The 1H NMR spectra of 1 and 2 at room temperature show a single set of proton signals that are in agreement with C4v-symmetry. At low temperatures the molecules exist as a mixture of boat conformations featuring slow exchange on the chemical shift time scale. An energy barrier (ΔG‡298) of 55.5 kJ/mol and 52.0 kJ/mol was calculated for the boat-to-boat exchange of 1 and 2, respectively. The results of the ROESY experiments performed at 193 K and computational modelling suggest that in solution the resorcinarene podand1 adopts similar conformation to that present in its crystal structure, whereas podand 2 populates more versatile range of conformations in solution. © 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.NEW FINDINGS What is the central question of this study? Cerebrovascular reactivity (CVR) is a common functional test to assess brain health. Impaired CVR has been associated with all-cause cardiovascular mortality. This study investigated whether the duration of the CO2 -stimulus and the time-point used for data extraction would alter the CVR outcome measure. What is the main finding and its importance? This study demonstrated CVR measures calculated from 1- and 2-minute CO2 -stimulus durations were significantly higher than CVR calculated from a 4-minute CO2 -stimulus. CVR calculated from the first 2-minutes of the CO2 -stimulus were significantly higher than CVR calculated from the final minute if the duration was ≥4-minutes. This study highlights the need for consistent methodological approaches. ABSTRACT Cerebrovascular reactivity to carbon dioxide (CVR) is a common functional test to assess brain vascular health, though conflicting age and fitness effects have been reported. Studies have used different dy-state time-points within each stimulus duration were different (p  less then  0.001, η² = 0.454); specifically, for 4-min (p = 0.001) and 5-min (p  less then  0.001), but not 2-min stimulus durations (p = 0.273). These findings demonstrate that methodological differences alter the CVR measure. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.BACKGROUND Protein deficits have been associated with longer intensive care unit (ICU) stays and increased mortality. Current view suggests if protein goals are met, meeting full energy targets may be less important and prevent deleterious effects of overfeeding. We proposed a very-high protein (VHP) enteral nutrition (EN) formula could provide adequate protein, without overfeeding energy, in the first week of critical illness. METHODS This was a retrospective study of medical/surgical ICU patients receiving EN exclusively for ≥5 days during the first week of ICU admission. Twenty participants received standard EN; 20 participants received the VHP-EN formula (1 kcal/mL, 37% protein). Protein and energy prescribed/received, gastrointestinal tolerance, and feeding interruptions were examined. RESULTS Forty ICU patients [average Acute Physiology and Chronic Health Evaluation II score of 20.1] were included. Protein prescribed and received was significantly higher in the VHP group vs the standard EN group (135.5 g/d ± 22.9 vs 111.4 g/d ± 25; P = .003 and 112.2 g/d ± 27.8 vs 81.7 g/d ± 16.7, respectively; P = .002). Energy prescribed and received was similar between groups (1696 kcal/d ± 402 vs 1893 kcal/d ± 341, respectively; P = .101 and 1520 kcal/d ± 346 vs 1506 ± 380 kcal/d; P = .901). There were no differences in EN tolerance (P = .065) or feeding interruptions (P = .336). CONCLUSIONS Use of a VHP formula in ICU patients resulted in higher protein intakes without overfeeding energy or use of modular protein in the first 5 days of exclusive EN. © 2020 American Society for Parenteral and Enteral Nutrition.Development of novel bioimaging materials that exhibit organelle specific accumulation continues to be at the forefront of research interests and efforts. Among the various subcellular organelles, mitochondria, which are found in the cytoplasm of eukaryotic cells, are of particular interest in relation to their vital function. To date, most molecular probes that target mitochondria utilise delocalised lipophilic cations such as triphenylphosphonium and pyridinium. However, the use of such charged motifs is known to be detrimental to the working function of the mitochondrial transmembrane potential and there remains a strong case for development of neutral mitochondrial fluorescent probes. Herein, we demonstrate for the first time the exploitation of diketopyrrolopyrrole-based chemistries for the realisation of a neutral fluorescent probe that exhibits organelle specific accumulation within the mitochondria at the nanomolar level. The synthesised probe, which bears a neutral triphenylphosphine oxide moiety, exhibits a large Stokes shift and high fluorescence quantum yield in water, both highly sought-after properties in the development of bioimaging agents.

55 mins ago


This heterogeneity in the process flow for eMERGE3 RoR reflects the "real world" of genomic medicine in which RoR procedures must be shaped by the needs of the patients and institutional environments.The present study aimed to investigate whether recreational volleyball organized as small-sided games could improve fitness and health profiles of middle-aged men after 10 weeks of training. Twenty-four healthy men aged 35-55 were randomized in a small-sided recreational volleyball group (RV = 12; age 44.7 ± 6.34 years; body mass index 25.85 ± 1.74) and control group (CON = 12; age 42.9 ± 8.72 years; body mass index 25.62 ± 1.48). The RV group carried out a volleyball training program, whereas the CON group continued their daily life activities during this period. The participants in the RV group performed 2/3 training sessions of 90 min per week. https://www.selleckchem.com/products/MG132.html Results from a repeated measure ANOVA indicated a significant group × time interaction for low-density lipoprotein (LDL) cholesterol (F = 6.776, p = 0.016, partial ƞ2 = 0.235) and for resting heart rate (F = 11.647, p = 0.002, partial ƞ2 = 0.346) in favor of the RV group. No significant changes were observed for body weight, body mass index, and diastolic blood pressure. Results for physical fitness indicated a significant interaction for Yo-Yo intermittent recovery test-level 2 (F = 11.648, p = 0.003, partial ƞ2 = 0.380), with no significant changes in both groups for handgrip strength. Recreational small-sided volleyball can be an effective training modality to stimulate a decrease in LDL cholesterol and resting HR with small improvements in cardiovascular fitness. Recreational volleyball played only two times per week shows positive cardiovascular fitness and health-related adaptations, which may contribute to the reduction of the risk of developing lifestyle diseases.Dysregulation of microtubules and tubulin homeostasis has been linked to developmental disorders, neurodegenerative diseases, and cancer. In general, both microtubule-stabilizing and destabilizing agents have been powerful tools for studies of microtubule cytoskeleton and as clinical agents in oncology. However, many cancers develop resistance to these agents, limiting their utility. We sought to address this by developing a different kind of agent tubulin-targeted small molecule degraders. Degraders (also known as proteolysis-targeting chimeras (PROTACs)) are compounds that recruit endogenous E3 ligases to a target of interest, resulting in the target's degradation. We developed and examined several series of α- and β-tubulin degraders, based on microtubule-destabilizing agents. Our results indicate, that although previously reported covalent tubulin binders led to tubulin degradation, in our hands, cereblon-recruiting PROTACs were not efficient. In summary, while we consider tubulin degraders to be valuable tools for studying the biology of tubulin homeostasis, it remains to be seen whether the PROTAC strategy can be applied to this target of high clinical relevance.Our objective was to examine the feasibility of hair cortisol concentrations (HCC) as a biomarker to predict clinical pregnancy outcomes and investigate its potential associations with perceived anxiety, resilience, and depressive symptoms. A total of 43 participants were assessed using HCC, the state trait anxiety inventory (STAI), resilience scale (RS), and the depression subscale of the symptom checklist 90-R (SCL-90-R). Participants were approached at their second consultation with the reproductive endocrinologist (T1), before scheduling their IVF cycle, and then 12 weeks after (T2), at their post-transfer visit with the study coordinators, before the human chorionic gonadotropin (HCG) pregnancy test. The logistic regression model revealed that HCC at T2 predicted 46% of a positive pregnancy test [R2 = 0.46, (ß = 0.11, p less then 0.05)]. Pregnant women had higher levels of resilience at T2 (M = 149.29; SD = 17.56) when compared with non-pregnant women at T2 (M = 119.96; SD = 21.71). Significant differences were found between both groups in depression at T2 (t = 3.13, p = 0.01) and resilience at T2 (t = -4.89, p = 0.01). HCC might be a promising biomarker to calculate the probability of pregnancy in women using assisted reproductive technologies (ART).Compared to heterogenous Ziegler-Natta systems (ZNS), ansa-metallocene catalysts for the industrial production of isotactic polypropylene feature a higher cost-to-performance balance. In particular, the C2-symmetric bis(indenyl) ansa-zirconocenes disclosed in the 1990s are complex to prepare, less stereo- and/or regioselective than ZNS, and lose performance at practical application temperatures. The golden era of these complexes, though, was before High Throughput Experimentation (HTE) could contribute significantly to their evolution. Herein, we illustrate a Quantitative Structure - Activity Relationship (QSAR) model trained on a robust and highly accurate HTE database. The clear-box QSAR model utilizes, in particular, a limited number of chemically intuitive 3D geometric descriptors that screen various regions of space in and around the catalytic pocket in a modular way thus enabling to quantify individual substituent contributions. The main focus of the paper is on the methodology, which should be of rather broad applicability in molecular organometallic catalysis. Then again, it is worth emphasizing that the specific application reported here led us to identify in a comparatively short time novel zirconocene catalysts rivaling or even outperforming all previous homologues which strongly indicates that the metallocene story is not over yet.Aerobic exercise is a core component of cardiac rehabilitation (CR). Leading organizations recommend that the exercise prescriptions should be based on a symptom limited baseline graded exercise test (GXT). However, recent evidence suggests that only ~30% of CR clinics perform baseline GXTs. Consequently, exercise prescriptions including exercise progression in CR are not following standard exercise prescription guidelines. Therefore, the purpose of this review is to provide clinicians with evidence-based techniques for prescribing exercise in the absence of a baseline GXT. Intensity indicators (e.g., heart rate, perceived exertion) are reviewed, along with special exercise considerations for various disease states (e.g., heart failure, peripheral artery disease, and coronary artery disease). Baseline exercise testing remains the gold standard approach for prescribing exercise among heart disease patients, however, clinicians must be prepared to safely develop and monitor patients when a baseline GXT is not performed.


From an early age, children develop stereotypes toward older adults leading to potential ageism. In young adults, ageism includes conversational changes, also known as elderspeak, characterized by louder, slower, and simplified speech. Although it has direct consequence on older adults, to date no studies have explored elderspeak in children. We invited 137 Belgian children aged 7-12 to take part in a guessing game through a dissimulated Skype session. The child had to make two female interlocutors in turn, one young (25 years old) and the other old (75), guess two different words each. During the session, children remained unaware of the real purpose of the game. Prosody, verbal fluency, and semantic content of their speech were measured using the Praat and VocabProfil software. The results, analyzed using mixed-design ANOVA, showed that children spoke louder to the older interlocutor and tended to judge her less competent to guess words than the younger participant. When the older person was second in turn, children engaged in lengthier and more detailed explanations. Unexpectedly, positive views on older people correlated with higher voice intensity, suggesting that the child's differential vocal attitudes may reflect benevolent ageism. In conclusion, significant speech accommodation can be detected in 7- to 12-year-old children when they speak to an older compared with a younger interlocutor. This accommodation is characterized by louder voice and lengthier explanations. © 2020 John Wiley & Sons Ltd.OBJECTIVES This study sought to investigate the relation between left ventricular end-diastolic pressure (LVEDP) and outcomes in patients undergoing percutaneous coronary intervention (PCI) for acute coronary syndromes (ACS). BACKGROUND Risk stratification in ACS patients is important. Data on the role of LVEDP in the prognostication of ACS patients are scarce. METHODS A total of 1,410 patients undergoing PCI for ACS and with available data on LVEDP were divided according to LVEDP tertiles (lowest tertile ≤13 mmHg, intermediate tertile 14-20 mmHg, and highest tertile >20 mmHg). The primary endpoint was all-cause mortality at a median follow-up of 246 [28-848] days. RESULTS Median LVEDP was 16 (11-22) mmHg. All-cause mortality was 2.8%, 4.5%, and 15.0% in the lowest, the intermediate, and the highest LVEDP tertile groups (p  less then  .001), respectively. Belonging to the highest LVEDP tertile was associated with an increased risk of all-cause mortality (adjusted hazard ratio [HR] = 2.66, 95% confidence interval [CI] [1.30, 5.47], p = .008). By receiver operating characteristic curve analysis, the optimal cut-off value for predicting all-cause mortality was 20 mmHg (sensitivity 68.3%, specificity 72.5%). There was no differential effect of LVEDP on mortality in patients with and without LV dysfunction (interaction p = .23) or ST-elevation myocardial infarction as index ACS event (interaction p = .86). CONCLUSIONS In patients undergoing PCI for ACS, LVEDP was independently related with mortality. Hence, LVEDP should be incorporated into early risk stratification and clinical decision making of ACS patients. © 2020 Wiley Periodicals, Inc.Considering the problems of high costs, low catalytic activity and selectivity in the metal-based catalysts for CO2 electroreduction, we apply boron-containing metal-free B2 S sheet as an alternative to the traditional metal-based catalysts. Reaction energy calculations identify the preferred "Formate" pathway for CO2 conversion to CH3 OH on B2 S, in which the thermodynamic energy barrier obtained by using the Computational Hydrogen Electrode model is 0.57 eV, and the kinetic energy barrier obtained by searching the transition states is 1.18 eV. Another possible reaction pathway, "RWGS+CO-hydro", is suppressed and the hydrogen evolution reaction (HER) side reaction is nonspontaneous. Compared to Cu(211) with the highest catalytic activity among all transition metals, B2 S sheet exhibits a better catalytic activity with a lower overpotential for CO2 reduction and a better selectivity that suppresses the non-target reaction. © 2020 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.BACKGROUND Peptide receptor radionuclide therapy (PRRT) is effective for treating midgut neuroendocrine tumors (NETs); however, incorporation of PRRT into routine practice in the U.S. is not well studied. Herein we analyze the first year of PRRT implementation to determine tolerance of PRRT and factors that increase risk of PRRT discontinuation. MATERIALS AND METHODS Medical records were reviewed and data were abstracted on all patients with NETs scheduled for PRRT during the first year of PRRT implementation at a U.S. NET referral center (August 2018 through July 2019). Logistic regression was used to identify factors associated with PRRT discontinuation. RESULTS Fifty-five patients (56% male) were scheduled for PRRT over the study period. The most common primary NET location was small bowel (47%), followed by pancreas (26%), and 84% of the NETs were World Health Organization grade 1 or 2. The cohort was heavily pretreated with somatostatin analog (SSA) therapy (98%), non-SSA systemic therapy (64%), primary 21% of individuals prematurely discontinued PRRT. Patients with baseline liver function test abnormalities were more likely to discontinue PRRT than patients with normal liver function tests, which should be taken into consideration when selecting treatment options for NETs. © AlphaMed Press 2020.Individuals in free-living animal populations generally differ substantially in reproductive success, lifespan and other fitness-related traits and the molecular mechanisms underlying this variation are poorly understood. Telomere length and dynamics are candidate traits explaining this variation, as long telomeres predict a higher survival probability and telomere loss has been shown to reflect experienced "life stress". https://www.selleckchem.com/products/bay-k-8644.html However, telomere dynamics among very long-lived species are unresolved. Additionally, it is generally not well understood how telomeres relate with reproductive success or sex. We measured telomere length and dynamics in erythrocytes to assess their relation to age, sex and reproduction in Cory's Shearwaters (Calonectris borealis), a long-lived seabird, in the context of a long-term study. Adult males had on average 231 bp longer telomeres than females independent of age. In females, telomere length changed relatively little with age, whereas male telomere length declined significantly. Telomere shortening within males from one year to the next was three times higher than the inter-annual shortening rate based on cross-sectional data of males.

Videos

07/15/2024

In today’s episode of Direct Impact, Rick Sanchez is joined by Dr. Wilmer Leon and Garland Nixon, co-hosts of ‘The Critical Hour’ on Radio Sputnik. Together they wrap up a week of topics from President Joe Biden’s repeated public stumbles and growing concerns over his physical and mental fitness. Rick also criticizes Biden’s recent comments that suggest a world leadership delusion, disrespecting global leaders forging new alliances. Additionally, the panel addresses media bias, showcasing a rare video of President Vladimir Putin with Indian Prime Minister Narendra Modi, and concludes with a spoof on Vice President Kamala Harris from Comedy Central.

09/18/2023

With over 50,000 self proclaimed fitness influencers across the globe, some share science-backed helpful tips, others promote fitness advice that’s misguided at best and dangerous at worst. But are people going too far for attention? An Indonesian fitness influencer died when performing what he called a "dangerous as hell" routine. On this episode of 360 View, Scottie Nell Hughes looks at how the fitness industry is playing out across the globe. We speak with influencer and author Oli London about how we can balance between being healthy and attention grabbing.

12/21/2021

Starting on January 15, 2022, individuals will be required to show proof of COVID-19 vaccination to enter certain indoor spaces like restaurants, fitness facilities, entertainment spaces, and certain meeting spaces in Boston.

The proof of vaccination requirement for indoor venues will go into effect starting Jan. 15 for everyone ages 12 and up. All patrons and employees will be required to show proof that they have received at least one dose of vaccine. Beginning Feb. 15, everyone will have to show proof of two doses. Starting on March 1, children over age 5 will be required to be vaccinated as well.

Will we begin to see a spike of relocation to what some are now deeming "free states" as lockdowns continue to progress?

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Videos

07/15/2024

In today’s episode of Direct Impact, Rick Sanchez is joined by Dr. Wilmer Leon and Garland Nixon, co-hosts of ‘The Critical Hour’ on Radio Sputnik. Together they wrap up a week of topics from President Joe Biden’s repeated public stumbles and growing concerns over his physical and mental fitness. Rick also criticizes Biden’s recent comments that suggest a world leadership delusion, disrespecting global leaders forging new alliances. Additionally, the panel addresses media bias, showcasing a rare video of President Vladimir Putin with Indian Prime Minister Narendra Modi, and concludes with a spoof on Vice President Kamala Harris from Comedy Central.

09/18/2023

With over 50,000 self proclaimed fitness influencers across the globe, some share science-backed helpful tips, others promote fitness advice that’s misguided at best and dangerous at worst. But are people going too far for attention? An Indonesian fitness influencer died when performing what he called a "dangerous as hell" routine. On this episode of 360 View, Scottie Nell Hughes looks at how the fitness industry is playing out across the globe. We speak with influencer and author Oli London about how we can balance between being healthy and attention grabbing.

12/21/2021

Starting on January 15, 2022, individuals will be required to show proof of COVID-19 vaccination to enter certain indoor spaces like restaurants, fitness facilities, entertainment spaces, and certain meeting spaces in Boston.

The proof of vaccination requirement for indoor venues will go into effect starting Jan. 15 for everyone ages 12 and up. All patrons and employees will be required to show proof that they have received at least one dose of vaccine. Beginning Feb. 15, everyone will have to show proof of two doses. Starting on March 1, children over age 5 will be required to be vaccinated as well.

Will we begin to see a spike of relocation to what some are now deeming "free states" as lockdowns continue to progress?

Posts

48 mins ago


We evaluated 35 lesions across the intraventricular septum (UPA, N = 17 vs BPA, N = 18). No difference in volume, linear dimensions or impedance drop was observed in this area between UPA and BPA. However, BPA required half RF time and showed an increased transmurality trend. We then analyzed 73 lesions from the endocardial side (UPA, N = 35 vs BPA, N = 38) and 50 from the epicardial side (UPA, N = 11 vs BPA N = 39) of the ventricular free walls. Lesion transmurality was markedly improved by BPA (P = .030, odds ratio, 23.73 [4.71,31.96]). Ventricular BPA lesions were significantly deeper on the epicardial side (P  less then  .0001) and endocardial side (P = .015). CONCLUSION Bipolar ablation is more likely to create transmural and epicardial lesions in the ventricle wall. Half the time is needed for the creation of comparably deep and large lesions. © 2020 Wiley Periodicals, Inc.In this study, the synthesis of tetramethoxy resorcinarene podands bearing p-toluene arms connected by -SO3- (1) and -CH2O- (2) linkers is presented. In the solid state, the resorcinarene podand 1 forms an intramolecular self-inclusion complex of the p-toluene group, whereas the resorcinarene podand 2 did not show self-inclusion. https://www.selleckchem.com/products/resatorvid.html The conformation of the flexible resorcinarene podands in solution was investigated by 1D and 2D NMR spectroscopic techniques using variable temperature experiments, as well as, with computational methods including conformational search and subsequent density functional theory (DFT) optimization for representative structures. The 1H NMR spectra of 1 and 2 at room temperature show a single set of proton signals that are in agreement with C4v-symmetry. At low temperatures the molecules exist as a mixture of boat conformations featuring slow exchange on the chemical shift time scale. An energy barrier (ΔG‡298) of 55.5 kJ/mol and 52.0 kJ/mol was calculated for the boat-to-boat exchange of 1 and 2, respectively. The results of the ROESY experiments performed at 193 K and computational modelling suggest that in solution the resorcinarene podand1 adopts similar conformation to that present in its crystal structure, whereas podand 2 populates more versatile range of conformations in solution. © 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.NEW FINDINGS What is the central question of this study? Cerebrovascular reactivity (CVR) is a common functional test to assess brain health. Impaired CVR has been associated with all-cause cardiovascular mortality. This study investigated whether the duration of the CO2 -stimulus and the time-point used for data extraction would alter the CVR outcome measure. What is the main finding and its importance? This study demonstrated CVR measures calculated from 1- and 2-minute CO2 -stimulus durations were significantly higher than CVR calculated from a 4-minute CO2 -stimulus. CVR calculated from the first 2-minutes of the CO2 -stimulus were significantly higher than CVR calculated from the final minute if the duration was ≥4-minutes. This study highlights the need for consistent methodological approaches. ABSTRACT Cerebrovascular reactivity to carbon dioxide (CVR) is a common functional test to assess brain vascular health, though conflicting age and fitness effects have been reported. Studies have used different dy-state time-points within each stimulus duration were different (p  less then  0.001, η² = 0.454); specifically, for 4-min (p = 0.001) and 5-min (p  less then  0.001), but not 2-min stimulus durations (p = 0.273). These findings demonstrate that methodological differences alter the CVR measure. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.BACKGROUND Protein deficits have been associated with longer intensive care unit (ICU) stays and increased mortality. Current view suggests if protein goals are met, meeting full energy targets may be less important and prevent deleterious effects of overfeeding. We proposed a very-high protein (VHP) enteral nutrition (EN) formula could provide adequate protein, without overfeeding energy, in the first week of critical illness. METHODS This was a retrospective study of medical/surgical ICU patients receiving EN exclusively for ≥5 days during the first week of ICU admission. Twenty participants received standard EN; 20 participants received the VHP-EN formula (1 kcal/mL, 37% protein). Protein and energy prescribed/received, gastrointestinal tolerance, and feeding interruptions were examined. RESULTS Forty ICU patients [average Acute Physiology and Chronic Health Evaluation II score of 20.1] were included. Protein prescribed and received was significantly higher in the VHP group vs the standard EN group (135.5 g/d ± 22.9 vs 111.4 g/d ± 25; P = .003 and 112.2 g/d ± 27.8 vs 81.7 g/d ± 16.7, respectively; P = .002). Energy prescribed and received was similar between groups (1696 kcal/d ± 402 vs 1893 kcal/d ± 341, respectively; P = .101 and 1520 kcal/d ± 346 vs 1506 ± 380 kcal/d; P = .901). There were no differences in EN tolerance (P = .065) or feeding interruptions (P = .336). CONCLUSIONS Use of a VHP formula in ICU patients resulted in higher protein intakes without overfeeding energy or use of modular protein in the first 5 days of exclusive EN. © 2020 American Society for Parenteral and Enteral Nutrition.Development of novel bioimaging materials that exhibit organelle specific accumulation continues to be at the forefront of research interests and efforts. Among the various subcellular organelles, mitochondria, which are found in the cytoplasm of eukaryotic cells, are of particular interest in relation to their vital function. To date, most molecular probes that target mitochondria utilise delocalised lipophilic cations such as triphenylphosphonium and pyridinium. However, the use of such charged motifs is known to be detrimental to the working function of the mitochondrial transmembrane potential and there remains a strong case for development of neutral mitochondrial fluorescent probes. Herein, we demonstrate for the first time the exploitation of diketopyrrolopyrrole-based chemistries for the realisation of a neutral fluorescent probe that exhibits organelle specific accumulation within the mitochondria at the nanomolar level. The synthesised probe, which bears a neutral triphenylphosphine oxide moiety, exhibits a large Stokes shift and high fluorescence quantum yield in water, both highly sought-after properties in the development of bioimaging agents.

55 mins ago


This heterogeneity in the process flow for eMERGE3 RoR reflects the "real world" of genomic medicine in which RoR procedures must be shaped by the needs of the patients and institutional environments.The present study aimed to investigate whether recreational volleyball organized as small-sided games could improve fitness and health profiles of middle-aged men after 10 weeks of training. Twenty-four healthy men aged 35-55 were randomized in a small-sided recreational volleyball group (RV = 12; age 44.7 ± 6.34 years; body mass index 25.85 ± 1.74) and control group (CON = 12; age 42.9 ± 8.72 years; body mass index 25.62 ± 1.48). The RV group carried out a volleyball training program, whereas the CON group continued their daily life activities during this period. The participants in the RV group performed 2/3 training sessions of 90 min per week. https://www.selleckchem.com/products/MG132.html Results from a repeated measure ANOVA indicated a significant group × time interaction for low-density lipoprotein (LDL) cholesterol (F = 6.776, p = 0.016, partial ƞ2 = 0.235) and for resting heart rate (F = 11.647, p = 0.002, partial ƞ2 = 0.346) in favor of the RV group. No significant changes were observed for body weight, body mass index, and diastolic blood pressure. Results for physical fitness indicated a significant interaction for Yo-Yo intermittent recovery test-level 2 (F = 11.648, p = 0.003, partial ƞ2 = 0.380), with no significant changes in both groups for handgrip strength. Recreational small-sided volleyball can be an effective training modality to stimulate a decrease in LDL cholesterol and resting HR with small improvements in cardiovascular fitness. Recreational volleyball played only two times per week shows positive cardiovascular fitness and health-related adaptations, which may contribute to the reduction of the risk of developing lifestyle diseases.Dysregulation of microtubules and tubulin homeostasis has been linked to developmental disorders, neurodegenerative diseases, and cancer. In general, both microtubule-stabilizing and destabilizing agents have been powerful tools for studies of microtubule cytoskeleton and as clinical agents in oncology. However, many cancers develop resistance to these agents, limiting their utility. We sought to address this by developing a different kind of agent tubulin-targeted small molecule degraders. Degraders (also known as proteolysis-targeting chimeras (PROTACs)) are compounds that recruit endogenous E3 ligases to a target of interest, resulting in the target's degradation. We developed and examined several series of α- and β-tubulin degraders, based on microtubule-destabilizing agents. Our results indicate, that although previously reported covalent tubulin binders led to tubulin degradation, in our hands, cereblon-recruiting PROTACs were not efficient. In summary, while we consider tubulin degraders to be valuable tools for studying the biology of tubulin homeostasis, it remains to be seen whether the PROTAC strategy can be applied to this target of high clinical relevance.Our objective was to examine the feasibility of hair cortisol concentrations (HCC) as a biomarker to predict clinical pregnancy outcomes and investigate its potential associations with perceived anxiety, resilience, and depressive symptoms. A total of 43 participants were assessed using HCC, the state trait anxiety inventory (STAI), resilience scale (RS), and the depression subscale of the symptom checklist 90-R (SCL-90-R). Participants were approached at their second consultation with the reproductive endocrinologist (T1), before scheduling their IVF cycle, and then 12 weeks after (T2), at their post-transfer visit with the study coordinators, before the human chorionic gonadotropin (HCG) pregnancy test. The logistic regression model revealed that HCC at T2 predicted 46% of a positive pregnancy test [R2 = 0.46, (ß = 0.11, p less then 0.05)]. Pregnant women had higher levels of resilience at T2 (M = 149.29; SD = 17.56) when compared with non-pregnant women at T2 (M = 119.96; SD = 21.71). Significant differences were found between both groups in depression at T2 (t = 3.13, p = 0.01) and resilience at T2 (t = -4.89, p = 0.01). HCC might be a promising biomarker to calculate the probability of pregnancy in women using assisted reproductive technologies (ART).Compared to heterogenous Ziegler-Natta systems (ZNS), ansa-metallocene catalysts for the industrial production of isotactic polypropylene feature a higher cost-to-performance balance. In particular, the C2-symmetric bis(indenyl) ansa-zirconocenes disclosed in the 1990s are complex to prepare, less stereo- and/or regioselective than ZNS, and lose performance at practical application temperatures. The golden era of these complexes, though, was before High Throughput Experimentation (HTE) could contribute significantly to their evolution. Herein, we illustrate a Quantitative Structure - Activity Relationship (QSAR) model trained on a robust and highly accurate HTE database. The clear-box QSAR model utilizes, in particular, a limited number of chemically intuitive 3D geometric descriptors that screen various regions of space in and around the catalytic pocket in a modular way thus enabling to quantify individual substituent contributions. The main focus of the paper is on the methodology, which should be of rather broad applicability in molecular organometallic catalysis. Then again, it is worth emphasizing that the specific application reported here led us to identify in a comparatively short time novel zirconocene catalysts rivaling or even outperforming all previous homologues which strongly indicates that the metallocene story is not over yet.Aerobic exercise is a core component of cardiac rehabilitation (CR). Leading organizations recommend that the exercise prescriptions should be based on a symptom limited baseline graded exercise test (GXT). However, recent evidence suggests that only ~30% of CR clinics perform baseline GXTs. Consequently, exercise prescriptions including exercise progression in CR are not following standard exercise prescription guidelines. Therefore, the purpose of this review is to provide clinicians with evidence-based techniques for prescribing exercise in the absence of a baseline GXT. Intensity indicators (e.g., heart rate, perceived exertion) are reviewed, along with special exercise considerations for various disease states (e.g., heart failure, peripheral artery disease, and coronary artery disease). Baseline exercise testing remains the gold standard approach for prescribing exercise among heart disease patients, however, clinicians must be prepared to safely develop and monitor patients when a baseline GXT is not performed.


From an early age, children develop stereotypes toward older adults leading to potential ageism. In young adults, ageism includes conversational changes, also known as elderspeak, characterized by louder, slower, and simplified speech. Although it has direct consequence on older adults, to date no studies have explored elderspeak in children. We invited 137 Belgian children aged 7-12 to take part in a guessing game through a dissimulated Skype session. The child had to make two female interlocutors in turn, one young (25 years old) and the other old (75), guess two different words each. During the session, children remained unaware of the real purpose of the game. Prosody, verbal fluency, and semantic content of their speech were measured using the Praat and VocabProfil software. The results, analyzed using mixed-design ANOVA, showed that children spoke louder to the older interlocutor and tended to judge her less competent to guess words than the younger participant. When the older person was second in turn, children engaged in lengthier and more detailed explanations. Unexpectedly, positive views on older people correlated with higher voice intensity, suggesting that the child's differential vocal attitudes may reflect benevolent ageism. In conclusion, significant speech accommodation can be detected in 7- to 12-year-old children when they speak to an older compared with a younger interlocutor. This accommodation is characterized by louder voice and lengthier explanations. © 2020 John Wiley & Sons Ltd.OBJECTIVES This study sought to investigate the relation between left ventricular end-diastolic pressure (LVEDP) and outcomes in patients undergoing percutaneous coronary intervention (PCI) for acute coronary syndromes (ACS). BACKGROUND Risk stratification in ACS patients is important. Data on the role of LVEDP in the prognostication of ACS patients are scarce. METHODS A total of 1,410 patients undergoing PCI for ACS and with available data on LVEDP were divided according to LVEDP tertiles (lowest tertile ≤13 mmHg, intermediate tertile 14-20 mmHg, and highest tertile >20 mmHg). The primary endpoint was all-cause mortality at a median follow-up of 246 [28-848] days. RESULTS Median LVEDP was 16 (11-22) mmHg. All-cause mortality was 2.8%, 4.5%, and 15.0% in the lowest, the intermediate, and the highest LVEDP tertile groups (p  less then  .001), respectively. Belonging to the highest LVEDP tertile was associated with an increased risk of all-cause mortality (adjusted hazard ratio [HR] = 2.66, 95% confidence interval [CI] [1.30, 5.47], p = .008). By receiver operating characteristic curve analysis, the optimal cut-off value for predicting all-cause mortality was 20 mmHg (sensitivity 68.3%, specificity 72.5%). There was no differential effect of LVEDP on mortality in patients with and without LV dysfunction (interaction p = .23) or ST-elevation myocardial infarction as index ACS event (interaction p = .86). CONCLUSIONS In patients undergoing PCI for ACS, LVEDP was independently related with mortality. Hence, LVEDP should be incorporated into early risk stratification and clinical decision making of ACS patients. © 2020 Wiley Periodicals, Inc.Considering the problems of high costs, low catalytic activity and selectivity in the metal-based catalysts for CO2 electroreduction, we apply boron-containing metal-free B2 S sheet as an alternative to the traditional metal-based catalysts. Reaction energy calculations identify the preferred "Formate" pathway for CO2 conversion to CH3 OH on B2 S, in which the thermodynamic energy barrier obtained by using the Computational Hydrogen Electrode model is 0.57 eV, and the kinetic energy barrier obtained by searching the transition states is 1.18 eV. Another possible reaction pathway, "RWGS+CO-hydro", is suppressed and the hydrogen evolution reaction (HER) side reaction is nonspontaneous. Compared to Cu(211) with the highest catalytic activity among all transition metals, B2 S sheet exhibits a better catalytic activity with a lower overpotential for CO2 reduction and a better selectivity that suppresses the non-target reaction. © 2020 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.BACKGROUND Peptide receptor radionuclide therapy (PRRT) is effective for treating midgut neuroendocrine tumors (NETs); however, incorporation of PRRT into routine practice in the U.S. is not well studied. Herein we analyze the first year of PRRT implementation to determine tolerance of PRRT and factors that increase risk of PRRT discontinuation. MATERIALS AND METHODS Medical records were reviewed and data were abstracted on all patients with NETs scheduled for PRRT during the first year of PRRT implementation at a U.S. NET referral center (August 2018 through July 2019). Logistic regression was used to identify factors associated with PRRT discontinuation. RESULTS Fifty-five patients (56% male) were scheduled for PRRT over the study period. The most common primary NET location was small bowel (47%), followed by pancreas (26%), and 84% of the NETs were World Health Organization grade 1 or 2. The cohort was heavily pretreated with somatostatin analog (SSA) therapy (98%), non-SSA systemic therapy (64%), primary 21% of individuals prematurely discontinued PRRT. Patients with baseline liver function test abnormalities were more likely to discontinue PRRT than patients with normal liver function tests, which should be taken into consideration when selecting treatment options for NETs. © AlphaMed Press 2020.Individuals in free-living animal populations generally differ substantially in reproductive success, lifespan and other fitness-related traits and the molecular mechanisms underlying this variation are poorly understood. Telomere length and dynamics are candidate traits explaining this variation, as long telomeres predict a higher survival probability and telomere loss has been shown to reflect experienced "life stress". https://www.selleckchem.com/products/bay-k-8644.html However, telomere dynamics among very long-lived species are unresolved. Additionally, it is generally not well understood how telomeres relate with reproductive success or sex. We measured telomere length and dynamics in erythrocytes to assess their relation to age, sex and reproduction in Cory's Shearwaters (Calonectris borealis), a long-lived seabird, in the context of a long-term study. Adult males had on average 231 bp longer telomeres than females independent of age. In females, telomere length changed relatively little with age, whereas male telomere length declined significantly. Telomere shortening within males from one year to the next was three times higher than the inter-annual shortening rate based on cross-sectional data of males.


To evaluate the imaging features and associations in patients with supravalvar aortic stenosis on multidetector computed tomography (CT) angiography.

We retrospectively reviewed all CT angiography studies performed for evaluation of congenital heart diseases at our institution through the period from January 2014 to June 2020. Cases with supravalvar aortic stenosis were identified and classified as syndromic and nonsyndromic based on history, physical examination, and relevant investigations. The type and extent of vascular involvement and associated cardiovascular abnormalities were characterized.

Supravalvar aortic stenosis was identified in 26/3926 (0.66%) patients (22 males and 4 females; Age range 2 months to 20 years). Discrete stenosis was seen in 14/26 (53.8%) patients, while diffuse involvement of the ascending aorta to varying degrees was seen in the remaining 12 (46.2%) patients. About 15/26 (57.7%) patients had pulmonary involvement at some level, namely,infundibular, valvar, supravalvar, orinfluence surgical outcomes.Incompletely reproductively isolated species often segregate into different microhabitats, even when they are able to survive and reproduce in both habitats. Longer term evolutionary factors may contribute to this lack of cross-habitat persistence. When reproductive interference reduces immigrant fitness, assortative mating, including self-fertilization, increases immigrants' fitness in a single generation, but longer term, inbreeding depression may reduce the chance of population persistence. Two California monkeyflower species repeatedly segregate into drier and wetter areas in their zone of sympatry. To test whether inbreeding depression may contribute to the maintenance of this segregation pattern, we transplanted outbred and successively inbred Mimulus guttatus and Mimulus nudatus into their native habitats and heterospecific habitats. We measured germination, survival, and seed set and found that recurrent selfing reduced all aspects of fitness in both species, most strongly in foreign habitats. A simulation model, parameterized from the transplant experiment, found that inbreeding reduced fitness to such an extent that sequentially inbred populations of either species would be unable to persist in heterospecific-occupied habitats in the absence of continued gene flow. These results demonstrate that individual immigrants are unlikely to form persistent populations and thus, inbreeding depression contributes to the absence of fine-scale coexistence in this species pair.The usefulness of cyclophosphamide (CP) in the treatment of multiple human malignancies and immunological diseases is hindered by the danger of developing nephrotoxicity. The toxic metabolites of CP are suggested to be responsible for oxidative stress resulted from the production of reactive oxygen species (ROS) and stimulation of lipid peroxidation. Nebivolol (NEB) is a third-generation selective B1 adrenoceptor antagonist, but it has also various pharmacological properties such as anti-inflammation, anti-apoptotic, and antioxidant activities. Thus, the present study aims to explore the potential protective effect of NEB against CP-induced nephrotoxicity. A cumulative dose of CP (75 mg/kg) was administered to albino rats by intraperitoneal injection. The protective effect of NEB was investigated by co-administration of NEB (10 mg/kg orally daily). Administration of NEB with CP significantly improved renal functions and reduced the oxidative renal changes induced by CP injection. Co-administration of NEB ameliorated apoptosis and inflammatory markers that were markedly exaggerated by CP. Our results indicated that NEB could be used as a protective agent against CP-induced nephrotoxicity.
Application of linear-quadratic (LQ) model to large fractional dose treatments is inconsistent with observed cell survival curves having a straight portion at high doses. We have proposed a unified multi-activation (UMA) model to fit cell survival curves over the entire dose range that allows us to calculate EQD2 for hypofractionated SBRT, SRT, SRS, and HDRB.

A unified formula of cell survival



S


=


n


/





e



D



D


o





+


n


-


1




using only the extrapolation number of n and the dose slope of D
was derived. Coefficient of determination, R
, relative residuals, r, and relative experimental errors, e, normalized to survival fraction at each dose point, were calculated to quantify the goodness in modeling of a survivl inactivation and can estimate EQD2 at all dose levels.
Our UMA model validated through many tumor cell lines can fit cell survival curves over the entire dose range within their experimental errors. https://www.selleckchem.com/products/nlg919.html The unified formula theoretically indicates a common mechanism of cell inactivation and can estimate EQD2 at all dose levels.Satellite-based remote sensing allows for global estimates of phytoplankton primary productivity by converting measurements of ocean color or photon absorption into units of carbon fixation. Models which perform this conversion often require an estimate of phytoplankton photoacclimation state such as the carbon to chlorophyll a ratio (CChl). Recently, our group developed a new photoacclimation model that can be applied to models of primary production. The model assumes that the phytoplankton photoacclimation state is not affected by periods of darkness during deep mixing beneath the photic zone, due to reduction in the plastoquinone pool in darkness and the subsequent deactivation of the signal for chlorophyll synthesis. In this study, we tested these assumptions by culturing the marine diatom Thalassiosira weissflogii under fluctuating light conditions simulating three different optical depths with progressively increasing deep mixing periods. The photoacclimation state, measured by the ratio of CChl, in T. weissflogii was not affected by changes in the length of simulated deep mixing periods. In addition, analysis of photosynthesis vs. irradiance (PE) curves showed that increases in optical depth caused decreases in both the maximum Chl-normalized rate of photosynthesis (Pb max ) and in the slope of light-limited photosynthesis (αb ), but had no effect on the half-saturation irradiance (Ek , another metric of photoacclimation). However, measurements of chlorophyll fluorescence during simulated deep mixing did not support the hypothesis that the PQ pool was reduced during dark periods. Thus, our findings support the use of the photoacclimation model for estimating primary production while suggesting the need for further research into the mechanisms controlling photoacclimation in the upper mixed layer environment of the ocean.

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5 T and  less then  12% at 3.0 T. The feasibility of MRF at 1.5 T and 3 T was demonstrated in the pancreas. By enabling fast and free-breathing quantitation, MRF has the potential to add value during the clinical characterisation and grading of pathological conditions, such as pancreatitis or cancer.Despite the accumulating evidences of the significance of humoral cancer immunity, its molecular mechanisms have largely remained elusive. Here we show that B-cell repertoire sequencing of 102 clinical gastric cancers and molecular biological analyses unexpectedly reveal that the major humoral cancer antigens are not case-specific neo-antigens but are rather commonly identified as ribonucleoproteins (RNPs) in the focal adhesion complex. These common antigens are shared as autoantigens with multiple autoimmune diseases, suggesting a direct molecular link between cancer- and auto-immunity on the focal adhesion RNP complex. This complex is partially exposed to the outside of cancer cell surfaces, which directly evokes humoral immunity and enables functional bindings of antibodies to cancer cell surfaces in physiological conditions. These findings shed light on humoral cancer immunity in that it commonly targets cellular components fundamental for cytoskeletal integrity and cell movement, pointing to a novel modality of immunotherapy using humoral immunological reactions to cancers.Xenotransplantation using pig organs has achieved survival times up to 195 days in pig orthotopic heart transplantation into baboons. Here we demonstrate that in addition to an improved immunosuppressive regimen, non-ischaemic preservation with continuous perfusion and control of post-transplantation growth of the transplant, prevention of transmission of the porcine cytomegalovirus (PCMV) plays an important role in achieving long survival times. For the first time we demonstrate that PCMV transmission in orthotopic pig heart xenotransplantation was associated with a reduced survival time of the transplant and increased levels of IL-6 and TNFα were found in the transplanted baboon. Furthermore, high levels of tPA-PAI-1 complexes were found, suggesting a complete loss of the pro-fibrinolytic properties of the endothelial cells. These data show that PCMV has an important impact on transplant survival and call for elimination of PCMV from donor pigs.Over the years, an amount of models relying on effective parameters were implemented in the challenging issue of the topside ionosphere description. These models are based on different analytical functions, but all of them depend on a parameter called effective scale height, that is deduced from topside electron density measurements. As their names state, they are effective in reproducing the topside electron density profile only when applied to the analytical function used to derive them. Then, in principle, they do not have any physical meaning. It is the goal of this paper to mathematically link the effective scale height modeled through the Epstein layer to the vertical scale height theoretically deduced from the plasma ambipolar diffusion theory. Firstly, effective and theoretical scale heights are linked through a mathematical relation by showing that they tend to each other in the topside ionosphere. https://www.selleckchem.com/products/ly333531.html Secondly, their connection is preliminarily demonstrated by calculating effective scale height values from the entire COSMIC/FORMOSAT-3 radio occultation dataset. Thirdly, a possible connection between the vertical gradient of the topside scale height (as obtained by COSMIC/FORMOSAT-3 satellites) and the electron temperature (as obtained by ESA Swarm B satellite) is studied by highlighting corresponding similarities in the diurnal, seasonal, solar activity, and latitudinal variability.The influence of kinship on animal cooperation is often unclear. Cooperating Asiatic lion coalitions are linearly hierarchical; male partners appropriate resources disproportionately. To investigate how kinship affect coalitionary dynamics, we combined microsatellite based genetic inferences with long-term genealogical records to measure relatedness between coalition partners of free-ranging lions in Gir, India. Large coalitions had higher likelihood of having sibling partners, while pairs were primarily unrelated. Fitness computations incorporating genetic relatedness revealed that low-ranking males in large coalitions were typically related to the dominant males and had fitness indices higher than single males, contrary to the previous understanding of this system based on indices derived from behavioural metrics alone. This demonstrates the indirect benefits to (related) males in large coalitions. Dominant males were found to 'lose less' if they lost mating opportunities to related partners versus unrelated males. From observations on territorial conflicts we show that while unrelated males cooperate, kin-selected benefits are ultimately essential for the maintenance of large coalitions. Although large coalitions maximised fitness as a group, demographic parameters limited their prevalence by restricting kin availability. Such demographic and behavioural constraints condition two-male coalitions to be the most attainable compromise for Gir lions.A novel design of thin-film crystalline silicon solar cell (TF C-Si-SC) is proposed and numerically analyzed. The reported SC has 1.0 µm thickness of C-Si with embedded dielectric silicon dioxide nanowires (NWs). The introduced NWs increase the light scattering in the active layer which improves the optical path length and hence the light absorption. The SC geometry has been optimized using particle swarm optimization (PSO) technique to improve the optical and electrical characteristics. The suggested TF C-Si-SC with two embedded NWs offers photocurrent density ([Formula see text]) of 32.8 mA cm-2 which is higher than 18 mA cm-2 of the conventional thin film SC with an enhancement of 82.2%. Further, a power conversion efficiency of 15.9% is achieved using the reported SC.Plastic debris in the ocean form a new ecosystem, termed 'plastisphere', which hosts a variety of marine organisms. Recent studies implemented DNA metabarcoding to characterize the taxonomic composition of the plastisphere in different areas of the world. In this study, we used a modified metabarcoding approach which was based on longer barcode sequences for the characterization of the plastisphere biota. We compared the microbiome of polyethylene food bags after 1 month at sea to the free-living biome in two proximal but environmentally different locations on the Mediterranean coast of Israel. We targeted the full 1.5 kb-long 16S rRNA gene for bacteria and 0.4-0.8 kb-long regions within the 18S rRNA, ITS, tufA and COI loci for eukaryotes. The taxonomic barcodes were sequenced using Oxford Nanopore Technology with multiplexing on a single MinION flow cell. We identified between 1249 and 2141 species in each of the plastic samples, of which 61 species (34 bacteria and 27 eukaryotes) were categorized as plastic-specific, including species that belong to known hydrocarbon-degrading genera.