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10/27/2024


If you do download software program, read the end user license agreement earlier than committing to the installation. As mentioned earlier, when you click on on the subsequent button to install software program, stating you could have read and understand the top user license agreement, you are permitting spyware to go to work in your pc. Macintosh computer systems have historically had very few problems with spyware or other malware infestations. Again, that's only if in case you have a stable protection bundle in place and are preserving it and your working system updated at all times. Taking motion to avoid getting spyware in your pc in the first place helps stop you from changing into a sufferer. The reason is straightforward: Windows computers make up greater than 90 p.c of systems in use, which means it's simply not definitely worth the effort and time required to develop spyware for the Mac OS. It's a good way to seek out out if you happen to'd like to stay in a international country and educate English for an extended period of time. Now is the time for singular focus. Now there's a new guessing game that takes the same structure and applies it to world geography.

The sport is dotted with bodily and logical puzzles, but it’s the emergence of emotional depth via dialog that stays within the player’s reminiscence, as Alex copes with the current lack of her brother whereas attempting to open as much as new relationships. While that is often a solo recreation, Live Sudoku and UsDoku each feature multi-player variations. Restoring a basic automotive is a phrase-of-mouth recreation, and specialty swap meets are the A-1 source for this type of knowledge. Aboriginal Australians are believed to have lived on their island continent for as long as 60,000 years. As long as you have crew spirit to share, you're welcome to include yourself at any pre-sport, parking lot occasion. Advanced air interfaces, resembling LTE, can use rather a lot of different frequencies, corresponding to seven hundred MHz, 900 MHz, 1.7 GHz, 2.1 GHz and dozens more. And, the higher your BATNA is, the stronger -- and more confident -- your negotiating may be. Install a firewall and, if you have a home community, use a separate router, moderately than sharing the Internet connection by means of certainly one of your computer systems.

Rare Space Lego units from the early 1980s have sold online for $5,000. Dungeons aren't essentially underground -- castles, space stations and even out of doors temples can all be labeled as dungeons. This is sort of sure to make the machine run faster and enhance the space accessible on your hard drive. Next, use your individual anti-virus software to run an entire scan of the system. Run a comprehensive scan using your anti-virus software program. Install an anti-virus software program package deal and keep it updated at all times. Updated anti-virus or anti-spyware software program will most likely catch the errant program, display a warning and take care of the issue for you. The Xbox 360, like all video recreation consoles, is just a computer with hardware and software program dedicated to the operate of running video game software program. When in doubt, don't install the software program. The issue is that many users really consent to spyware being included when they obtain and install free utilities or video games. When a number of competing consoles provide the identical standard recreation but that game is missing from Sega’s library, that’s an issue.

Note that the Xbox will store as much as 10 minutes of recreation play, or up to an hour for those who join an external laborious drive. These MMOGs are free to play, but players may optionally buy in-sport objects or forex. If you're on the lookout for anti-virus software program, go on to the company's Web site and be certain you're where you assume you might be. I'm an entertaining speaker (not that you'd understand it from this internet web page). For extra great articles on the impression of Tv on society, try the links on the subsequent page. For extra info on viruses, spyware, Trojans and different nefarious software program, scan over to the next web page. You may see just a few more warning messages, but it is a small worth to pay for safety. Sony's community stayed down for weeks as it performed a criminal investigation into the hacking incident and rebuilt its infrastructure to extend security. While the number of threats to computer systems appears to extend nearly daily, the protection mechanisms obtainable to guard them are also growing in number and sophistication. While increased https://pafikabkotabontang.org/ of mental well being issues is mostly a great factor, it falls decidedly flat when phrases like gaslighting, ADHD, OCD and such are grossly misused.

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10/18/2024

Swing state storms and pre-election denialism now mass-lighting every Democrats’ dreams and nightmares at the same time

https://newstarget.com/2024-10-17-misinformation-industrial-complex-gaslighting-americans-climate-change.html

The hypocrisy from the Left is deafening. If ever there have been true election deniers, it is Congressmen on the Left already saying right now, before the 2024 election has even taken place, that they will not certify a Donald J. Trump win. Is this pre-election insurrection? Should they be put in prison in the […]

www.newstarget.com

10/06/2024


Hyoscyamine (HYO) and scopolamine (SCO) are tropane alkaloids acting as anticholinergic factors on the parasympathetic nervous system in humans and are produced by Solanaceous plants. Two strains of Agrobacterium rhizogenes, A4 and LBA9402, were used to infect Atropa acuminata Royle ex Miers and Atropa belladonna L. leaf explants. A. acuminata was inoculated either by direct infection or sonicated-assisted A. rhizogenes-mediated transformation (SAAT) was performed. A. belladonna was inoculated with the A4 strain using a direct method. The selected hairy root lines of both species were elicited with 50 mM methyl-β-cyclodextrin (β-CD), 0.5 μM coronatine (Cor) or 50 mM β-CD + 0.5 μM Cor on Day 14 of culture. The elicitor effect on growth and HYO and SCO content was analyzed after one (T1) and two (T2) weeks of treatment. In A. acuminata explants, the highest transformation percentage (T%) was obtained with strain A4 and the SAAT method (T% 96.43). Cor significantly reduced the growth of A. acuminata hairy roots (fresh weight and dry weight [DW] 2.52 and 0.3 g, respectively), whereas β-CD increased their DW (0.4 g). Also, the combined β-CD + Cor treatment had a positive significant effect on the DW of A. belladonna hairy roots (0.41 g). In A. acuminata hairy roots, the HYO level was lower under Cor treatment than in the control at both sampling times. In contrast, the SCO content was increased 10-fold by Cor elicitation at T1 compared to the control (10.95 mg g-1 DW) and was also positively affected by  β-CD + Cor. In A. belladonna hairy roots, all the elicitors had a negative effect on both HYO and SCO production. This report is the first assessment of the effect of β-CD and Cor elicitors on tropane alkaloid production.The extinction of species is a non-random process, and understanding why some species are more likely to go extinct than others is critical for conservation efforts. Functional trait-based approaches offer a promising tool to achieve this goal. In forests, deadwood-dependent (saproxylic) beetles comprise a major part of threatened species, but analyses of their extinction risk have been hindered by the availability of suitable morphological traits. To better understand the mechanisms underlying extinction in insects, we investigated the relationships between morphological features and the extinction risk of saproxylic beetles. Specifically, we hypothesised that species darker in colour, with a larger and rounder body, a lower mobility, lower sensory perception and more robust mandibles are at higher risk. We first developed a protocol for morphological trait measurements and present a database of 37 traits for 1,157 European saproxylic beetle species. Based on 13 selected, independent traits characterising aspidly growing field of ecological and conservation studies of deadwood.Camera traps are an increasingly popular tool to monitor wildlife distributions. However, traditional analytical approaches to camera trap data are difficult to apply to visible wildlife characteristics in single images, such as infection status. Several parasites produce visible signs of infection that could be sampled via camera traps. Sarcoptic mange Sarcoptes scabiei is an ideal disease to study using cameras because it results in visible hair loss and affects a broad host range. Here, we developed a multi-state occupancy model to estimate the occurrence of mange in coyotes Canis latrans across an urban gradient. This model incorporates a secondary detection function for apparent by-image infection status to provide detection-corrected estimates of mange occurrence. We analysed a multi-year camera trap dataset in Chicago, Illinois, United States, to test whether the apparent occurrence of sarcoptic mange in coyotes Canis latrans increases with urbanization or varies through time. We documented visible sigse. More generally, our model could provide detection-corrected occupancy estimates of visible characteristics that vary by image such as body condition or injuries.Gaslighting is an insidious, pernicious form of bullying that is gaining notoriety in nursing academia anecdotally. https://www.selleckchem.com/products/ABT-888.html Attempting to identify the behaviors associated with gaslighting are difficult because of the subtle nature with which gaslighting is performed by the perpetrator. Traditional tools/models used for identifying bullying and horizontal violence are not sensitive enough to pick up gaslighting behaviors. Perpetual lies and mistruths, vexatious or frivolous complaints, praise and positive reinforcement, the use of blame or mobbing, and coercion are some of the tactics a gaslighter will use to create confusion and chaos for the gaslightee. The psychological harm for the gaslightee can lead to self-doubt, hypervigilance, depression, anxiety, addiction, and suicidal ideation as well as the physical symptoms associated with stress. Left with very few choices the gaslightee either adheres to the behavior or leaves. It is important to raise awareness and perhaps education and training of this form of bullying behavior because of the deleterious effects it has personally and professionally.Climate change may have dramatic consequences for communities through both direct effects of peak temperatures upon individual species and through interspecific mismatches in thermal sensitivities of interacting organisms which mediate changes in interspecific interactions (i.e. predation). Despite this, there is a paucity of information on the patterns of spatial physiological sensitivity of interacting species (at both landscape and local scales) which could ultimately influence geographical variation in the effects of climate change on community processes. In order to assess where these impacts may occur, we first need to evaluate the spatial heterogeneity in the degree of mismatch in thermal tolerances between interacting organisms. We quantify the magnitude of interspecific mismatch in maximum (CTmax ) and minimum (CTmin ) thermal tolerances among a predator-prey system of dragonfly and anuran larvae in tropical montane (242-3,631 m) and habitat (ponds and streams) gradients. To compare thermal mismatchey naiad predators may further increase the vulnerability of lowland tadpoles to warming due to potentially enhanced indirect effects of higher predation rates by more heat-tolerant dragonfly predators. However, further experimental work is required to establish the individual and population-level consequences of this thermal tolerance mismatch upon biotic interactions such as predator-prey. .
Microaggressions, a concept originally introduced for ethnic minorities, represent subtle day-to-day discrimination, damaging the psychological health and well-being of lesbian, gay, bisexual, and transgender individuals as well. This study aimed to assess whether microaggressions occur in psychotherapists' assessments of clients who identify as either lesbian or transgender woman when compared with those identifying as heterosexual woman.

The study included a sample of 135 licensed psychotherapists (110 cisgender women and 25 cisgender men). Participants were presented with an audio file of a woman introducing herself during her first therapy session. Three versions were presented a transgender, a lesbian, and a heterosexual client. Participants were asked to assess the clinical relevance of 10 questions defined as neutral (N = 5) and microaggressive (N = 5), used to determine a clinical impression of the client. A repeated measure analysis of variance was conducted to understand the likelihood of clients of different gender identity and sexual orientation receiving microaggressions.

Participants were more prone to consider microaggressive questions relevant where the client identified as either lesbian or transgender.

The findings highlighted a bias against lesbian and trans women in evaluating the relevance and usefulness of clinical information while making a psychological assessment of a case.
The findings highlighted a bias against lesbian and trans women in evaluating the relevance and usefulness of clinical information while making a psychological assessment of a case.Understanding the forces that shape species distributions is increasingly important in a fast-changing world. Although major disturbance events can adversely affect natural populations, they can also present new opportunities, for example by opening up habitat for colonization by other lineages. Following extensive geographic sampling, we use genomic data to infer a range extension following disturbance for an ecologically important intertidal macroalgal species. Specifically, we genotyped 288 southern bull kelp (Durvillaea) plants from 28 localities across central New Zealand. All specimens from the North Island were expected to be D. antarctica, but unexpectedly 10 samples from four sites were identified as D. poha. Extensive sampling from the northern South Island (105 samples at five locations) confirmed the absence of D. poha north of the Kaikōura Peninsula. The North Island specimens of D. poha therefore reveal a biogeographic disjunction, some 150 km northeast of the nearest (South Island) population of this species. Based on strong geographic correspondence between these North Island samples and historic disturbance, we infer that tectonic upheaval, particularly earthquake-generated landslides, likely extirpated local D. antarctica and created an opportunity for a northward range expansion event by D. poha. Close phylogenomic relationships between this new North Island population and South Island samples support a geologically recent northward expansion, rather than a deeper evolutionary origin. These findings indicate the potential of large-scale disturbances to facilitate sudden biogeographic range expansions, and they emphasize the ability of genomic analyses with fine-scale sampling to reveal long-lasting signatures of past disturbance, dispersal, and colonization.Infectious diseases affecting habitat-forming species can have significant impacts on population dynamics and alter the structure and functioning of marine ecosystems. Recently, a fungal infection was described as the causative agent of necrotic lesions on the stipe of the forest-forming macroalga Phyllospora comosa, a disease named "stipe rot" (SR). Here, we developed a quantitative PCR (qPCR) method for rapid detection and quantification of this pathogen, which was applied to evaluate the level of SR infection in eight P. comosa populations spanning the entire latitudinal distribution of this species along southeastern Australia. We also investigated the relationship between the abundance and prevalence of Stipe Rot Fungus (SRF) and potential host chemical defenses as well as its relationship with morphological and ecophysiological traits of P. comosa. qPCR estimates of SRF abundance reflected the levels of infection estimated by visual assessment, with higher numbers of SRF copies being observed in individuals showing high or intermediate levels of visual symptoms of SR. Concordance of conventional PCR and visual assessments was 92 and 94%, respectively, compared to qPCR detection. SRF prevalence was positively related to fucoxanthin content and herbivory, but not significant related to other traits measured (phlorotannin content, total length, thallus diameter, stipe width, number of branches, frond width, fouling, bleaching, gender, and photosynthetic efficiency). These results provide confidence for previous reports of this disease based upon visual assessments only, contribute to the development of monitoring and conservation strategies for safeguarding P. comosa forests, and generate insights into potential factors influencing host-pathogen interactions in this system.

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The US government needs to borrow another $2 trillion (or more) in 2024, but has just announced they will be buying their own debt back in 2024. What’s the real story here?
In this episode of Finance University Pual and I discuss the recent Honey Badger Gathering, as well as the extreme gaslighting by the US Treasury Department which is planning to buy back(!) Treasuries in order to “reduce volatility in their cash balances.”

Check out PeakFinancialInvesting.com today!

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Elias showed typical milestones and growth until his 4 month “Wellness” visit. Despite Elias having a cold, the doctor gave him 4 vaccines that day. At 4 a.m. following the visit, Elias began having seizures.

After years of seizures, medical gaslighting, and his parents searching for answers, Elias unfortunately passed away at 4 years old after a 75 minute seizure.

Elias’s photograph is now placed in-memoriam at the front of the #CHDBus, where CHD will be collecting vaccine death and injury stories from across the country.

Watch the full story on #CHDTV ?

https://live.childrenshealthdefense.org/chd-tv/shows/good-morning-chd/dr-mercola-responds-to-chase-debanking--more/

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The US government needs to borrow another $2 trillion (or more) in 2024, but has just announced they will be buying their own debt back in 2024. What’s the real story here?
In this episode of Finance University Pual and I discuss the recent Honey Badger Gathering, as well as the extreme gaslighting by the US Treasury Department which is planning to buy back(!) Treasuries in order to “reduce volatility in their cash balances.”

Check out PeakFinancialInvesting.com today!

Order THE CRASH COURSE here:
https://www.barnesandnoble.com/w/the-...

Wanna buy me a coffee? https://www.buymeacoffee.com/PeakPros...

Join the #1 resilience community today!
https://peakprosperity.com/membership/

ALSO FOLLOW US HERE:

Twitter: @Chris_martenson

https://rumble.com/c/PeakProsperity

https://odysee.com/@Chris_Martenson:2

FINANCIAL DISCLAIMER. PEAK PROSPERITY, LLC, AND PEAK FINANCIAL INVESTING ARE NOT ENGAGED IN RENDERING LEGAL, TAX, OR FINANCIAL ADVICE OR SERVICES VIA THIS WEBSITE. NEITHER PEAK PROSPERITY, LLC NOT PEAK FINANCIAL INVESTING ARE FINANCIAL PLANNERS, BROKERS, OR TAX ADVISORS. Their websites are intended only to assist you in your financial education. Your personal financial situation is unique, and any information and advice obtained through this website may not be appropriate for your situation. Accordingly, before making any final decisions or implementing any financial strategy, you should consider obtaining additional information and advice from your accountant or other financial advisers who are fully aware of your individual circumstances.

Elias showed typical milestones and growth until his 4 month “Wellness” visit. Despite Elias having a cold, the doctor gave him 4 vaccines that day. At 4 a.m. following the visit, Elias began having seizures.

After years of seizures, medical gaslighting, and his parents searching for answers, Elias unfortunately passed away at 4 years old after a 75 minute seizure.

Elias’s photograph is now placed in-memoriam at the front of the #CHDBus, where CHD will be collecting vaccine death and injury stories from across the country.

Watch the full story on #CHDTV ?

https://live.childrenshealthdefense.org/chd-tv/shows/good-morning-chd/dr-mercola-responds-to-chase-debanking--more/

Welcome to The Daily Wrap Up, a concise show dedicated to bringing you the most relevant independent news, as we see it, from the last 24 hours.

All Video Source Links Can Be Found Here At The Last American Vagabond: https://www.thelastamericanvagabond.com/gaslighting-child-trafficking

Want to send a check to support TLAV, or just words of encouragement?
Use our new P.O. box:
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10/27/2024


If you do download software program, read the end user license agreement earlier than committing to the installation. As mentioned earlier, when you click on on the subsequent button to install software program, stating you could have read and understand the top user license agreement, you are permitting spyware to go to work in your pc. Macintosh computer systems have historically had very few problems with spyware or other malware infestations. Again, that's only if in case you have a stable protection bundle in place and are preserving it and your working system updated at all times. Taking motion to avoid getting spyware in your pc in the first place helps stop you from changing into a sufferer. The reason is straightforward: Windows computers make up greater than 90 p.c of systems in use, which means it's simply not definitely worth the effort and time required to develop spyware for the Mac OS. It's a good way to seek out out if you happen to'd like to stay in a international country and educate English for an extended period of time. Now is the time for singular focus. Now there's a new guessing game that takes the same structure and applies it to world geography.

The sport is dotted with bodily and logical puzzles, but it’s the emergence of emotional depth via dialog that stays within the player’s reminiscence, as Alex copes with the current lack of her brother whereas attempting to open as much as new relationships. While that is often a solo recreation, Live Sudoku and UsDoku each feature multi-player variations. Restoring a basic automotive is a phrase-of-mouth recreation, and specialty swap meets are the A-1 source for this type of knowledge. Aboriginal Australians are believed to have lived on their island continent for as long as 60,000 years. As long as you have crew spirit to share, you're welcome to include yourself at any pre-sport, parking lot occasion. Advanced air interfaces, resembling LTE, can use rather a lot of different frequencies, corresponding to seven hundred MHz, 900 MHz, 1.7 GHz, 2.1 GHz and dozens more. And, the higher your BATNA is, the stronger -- and more confident -- your negotiating may be. Install a firewall and, if you have a home community, use a separate router, moderately than sharing the Internet connection by means of certainly one of your computer systems.

Rare Space Lego units from the early 1980s have sold online for $5,000. Dungeons aren't essentially underground -- castles, space stations and even out of doors temples can all be labeled as dungeons. This is sort of sure to make the machine run faster and enhance the space accessible on your hard drive. Next, use your individual anti-virus software to run an entire scan of the system. Run a comprehensive scan using your anti-virus software program. Install an anti-virus software program package deal and keep it updated at all times. Updated anti-virus or anti-spyware software program will most likely catch the errant program, display a warning and take care of the issue for you. The Xbox 360, like all video recreation consoles, is just a computer with hardware and software program dedicated to the operate of running video game software program. When in doubt, don't install the software program. The issue is that many users really consent to spyware being included when they obtain and install free utilities or video games. When a number of competing consoles provide the identical standard recreation but that game is missing from Sega’s library, that’s an issue.

Note that the Xbox will store as much as 10 minutes of recreation play, or up to an hour for those who join an external laborious drive. These MMOGs are free to play, but players may optionally buy in-sport objects or forex. If you're on the lookout for anti-virus software program, go on to the company's Web site and be certain you're where you assume you might be. I'm an entertaining speaker (not that you'd understand it from this internet web page). For extra great articles on the impression of Tv on society, try the links on the subsequent page. For extra info on viruses, spyware, Trojans and different nefarious software program, scan over to the next web page. You may see just a few more warning messages, but it is a small worth to pay for safety. Sony's community stayed down for weeks as it performed a criminal investigation into the hacking incident and rebuilt its infrastructure to extend security. While the number of threats to computer systems appears to extend nearly daily, the protection mechanisms obtainable to guard them are also growing in number and sophistication. While increased https://pafikabkotabontang.org/ of mental well being issues is mostly a great factor, it falls decidedly flat when phrases like gaslighting, ADHD, OCD and such are grossly misused.

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pafikabkotabontang.org

10/18/2024

Swing state storms and pre-election denialism now mass-lighting every Democrats’ dreams and nightmares at the same time

https://newstarget.com/2024-10-17-misinformation-industrial-complex-gaslighting-americans-climate-change.html

The hypocrisy from the Left is deafening. If ever there have been true election deniers, it is Congressmen on the Left already saying right now, before the 2024 election has even taken place, that they will not certify a Donald J. Trump win. Is this pre-election insurrection? Should they be put in prison in the […]

www.newstarget.com

10/06/2024


Hyoscyamine (HYO) and scopolamine (SCO) are tropane alkaloids acting as anticholinergic factors on the parasympathetic nervous system in humans and are produced by Solanaceous plants. Two strains of Agrobacterium rhizogenes, A4 and LBA9402, were used to infect Atropa acuminata Royle ex Miers and Atropa belladonna L. leaf explants. A. acuminata was inoculated either by direct infection or sonicated-assisted A. rhizogenes-mediated transformation (SAAT) was performed. A. belladonna was inoculated with the A4 strain using a direct method. The selected hairy root lines of both species were elicited with 50 mM methyl-β-cyclodextrin (β-CD), 0.5 μM coronatine (Cor) or 50 mM β-CD + 0.5 μM Cor on Day 14 of culture. The elicitor effect on growth and HYO and SCO content was analyzed after one (T1) and two (T2) weeks of treatment. In A. acuminata explants, the highest transformation percentage (T%) was obtained with strain A4 and the SAAT method (T% 96.43). Cor significantly reduced the growth of A. acuminata hairy roots (fresh weight and dry weight [DW] 2.52 and 0.3 g, respectively), whereas β-CD increased their DW (0.4 g). Also, the combined β-CD + Cor treatment had a positive significant effect on the DW of A. belladonna hairy roots (0.41 g). In A. acuminata hairy roots, the HYO level was lower under Cor treatment than in the control at both sampling times. In contrast, the SCO content was increased 10-fold by Cor elicitation at T1 compared to the control (10.95 mg g-1 DW) and was also positively affected by  β-CD + Cor. In A. belladonna hairy roots, all the elicitors had a negative effect on both HYO and SCO production. This report is the first assessment of the effect of β-CD and Cor elicitors on tropane alkaloid production.The extinction of species is a non-random process, and understanding why some species are more likely to go extinct than others is critical for conservation efforts. Functional trait-based approaches offer a promising tool to achieve this goal. In forests, deadwood-dependent (saproxylic) beetles comprise a major part of threatened species, but analyses of their extinction risk have been hindered by the availability of suitable morphological traits. To better understand the mechanisms underlying extinction in insects, we investigated the relationships between morphological features and the extinction risk of saproxylic beetles. Specifically, we hypothesised that species darker in colour, with a larger and rounder body, a lower mobility, lower sensory perception and more robust mandibles are at higher risk. We first developed a protocol for morphological trait measurements and present a database of 37 traits for 1,157 European saproxylic beetle species. Based on 13 selected, independent traits characterising aspidly growing field of ecological and conservation studies of deadwood.Camera traps are an increasingly popular tool to monitor wildlife distributions. However, traditional analytical approaches to camera trap data are difficult to apply to visible wildlife characteristics in single images, such as infection status. Several parasites produce visible signs of infection that could be sampled via camera traps. Sarcoptic mange Sarcoptes scabiei is an ideal disease to study using cameras because it results in visible hair loss and affects a broad host range. Here, we developed a multi-state occupancy model to estimate the occurrence of mange in coyotes Canis latrans across an urban gradient. This model incorporates a secondary detection function for apparent by-image infection status to provide detection-corrected estimates of mange occurrence. We analysed a multi-year camera trap dataset in Chicago, Illinois, United States, to test whether the apparent occurrence of sarcoptic mange in coyotes Canis latrans increases with urbanization or varies through time. We documented visible sigse. More generally, our model could provide detection-corrected occupancy estimates of visible characteristics that vary by image such as body condition or injuries.Gaslighting is an insidious, pernicious form of bullying that is gaining notoriety in nursing academia anecdotally. https://www.selleckchem.com/products/ABT-888.html Attempting to identify the behaviors associated with gaslighting are difficult because of the subtle nature with which gaslighting is performed by the perpetrator. Traditional tools/models used for identifying bullying and horizontal violence are not sensitive enough to pick up gaslighting behaviors. Perpetual lies and mistruths, vexatious or frivolous complaints, praise and positive reinforcement, the use of blame or mobbing, and coercion are some of the tactics a gaslighter will use to create confusion and chaos for the gaslightee. The psychological harm for the gaslightee can lead to self-doubt, hypervigilance, depression, anxiety, addiction, and suicidal ideation as well as the physical symptoms associated with stress. Left with very few choices the gaslightee either adheres to the behavior or leaves. It is important to raise awareness and perhaps education and training of this form of bullying behavior because of the deleterious effects it has personally and professionally.Climate change may have dramatic consequences for communities through both direct effects of peak temperatures upon individual species and through interspecific mismatches in thermal sensitivities of interacting organisms which mediate changes in interspecific interactions (i.e. predation). Despite this, there is a paucity of information on the patterns of spatial physiological sensitivity of interacting species (at both landscape and local scales) which could ultimately influence geographical variation in the effects of climate change on community processes. In order to assess where these impacts may occur, we first need to evaluate the spatial heterogeneity in the degree of mismatch in thermal tolerances between interacting organisms. We quantify the magnitude of interspecific mismatch in maximum (CTmax ) and minimum (CTmin ) thermal tolerances among a predator-prey system of dragonfly and anuran larvae in tropical montane (242-3,631 m) and habitat (ponds and streams) gradients. To compare thermal mismatchey naiad predators may further increase the vulnerability of lowland tadpoles to warming due to potentially enhanced indirect effects of higher predation rates by more heat-tolerant dragonfly predators. However, further experimental work is required to establish the individual and population-level consequences of this thermal tolerance mismatch upon biotic interactions such as predator-prey. .
Microaggressions, a concept originally introduced for ethnic minorities, represent subtle day-to-day discrimination, damaging the psychological health and well-being of lesbian, gay, bisexual, and transgender individuals as well. This study aimed to assess whether microaggressions occur in psychotherapists' assessments of clients who identify as either lesbian or transgender woman when compared with those identifying as heterosexual woman.

The study included a sample of 135 licensed psychotherapists (110 cisgender women and 25 cisgender men). Participants were presented with an audio file of a woman introducing herself during her first therapy session. Three versions were presented a transgender, a lesbian, and a heterosexual client. Participants were asked to assess the clinical relevance of 10 questions defined as neutral (N = 5) and microaggressive (N = 5), used to determine a clinical impression of the client. A repeated measure analysis of variance was conducted to understand the likelihood of clients of different gender identity and sexual orientation receiving microaggressions.

Participants were more prone to consider microaggressive questions relevant where the client identified as either lesbian or transgender.

The findings highlighted a bias against lesbian and trans women in evaluating the relevance and usefulness of clinical information while making a psychological assessment of a case.
The findings highlighted a bias against lesbian and trans women in evaluating the relevance and usefulness of clinical information while making a psychological assessment of a case.Understanding the forces that shape species distributions is increasingly important in a fast-changing world. Although major disturbance events can adversely affect natural populations, they can also present new opportunities, for example by opening up habitat for colonization by other lineages. Following extensive geographic sampling, we use genomic data to infer a range extension following disturbance for an ecologically important intertidal macroalgal species. Specifically, we genotyped 288 southern bull kelp (Durvillaea) plants from 28 localities across central New Zealand. All specimens from the North Island were expected to be D. antarctica, but unexpectedly 10 samples from four sites were identified as D. poha. Extensive sampling from the northern South Island (105 samples at five locations) confirmed the absence of D. poha north of the Kaikōura Peninsula. The North Island specimens of D. poha therefore reveal a biogeographic disjunction, some 150 km northeast of the nearest (South Island) population of this species. Based on strong geographic correspondence between these North Island samples and historic disturbance, we infer that tectonic upheaval, particularly earthquake-generated landslides, likely extirpated local D. antarctica and created an opportunity for a northward range expansion event by D. poha. Close phylogenomic relationships between this new North Island population and South Island samples support a geologically recent northward expansion, rather than a deeper evolutionary origin. These findings indicate the potential of large-scale disturbances to facilitate sudden biogeographic range expansions, and they emphasize the ability of genomic analyses with fine-scale sampling to reveal long-lasting signatures of past disturbance, dispersal, and colonization.Infectious diseases affecting habitat-forming species can have significant impacts on population dynamics and alter the structure and functioning of marine ecosystems. Recently, a fungal infection was described as the causative agent of necrotic lesions on the stipe of the forest-forming macroalga Phyllospora comosa, a disease named "stipe rot" (SR). Here, we developed a quantitative PCR (qPCR) method for rapid detection and quantification of this pathogen, which was applied to evaluate the level of SR infection in eight P. comosa populations spanning the entire latitudinal distribution of this species along southeastern Australia. We also investigated the relationship between the abundance and prevalence of Stipe Rot Fungus (SRF) and potential host chemical defenses as well as its relationship with morphological and ecophysiological traits of P. comosa. qPCR estimates of SRF abundance reflected the levels of infection estimated by visual assessment, with higher numbers of SRF copies being observed in individuals showing high or intermediate levels of visual symptoms of SR. Concordance of conventional PCR and visual assessments was 92 and 94%, respectively, compared to qPCR detection. SRF prevalence was positively related to fucoxanthin content and herbivory, but not significant related to other traits measured (phlorotannin content, total length, thallus diameter, stipe width, number of branches, frond width, fouling, bleaching, gender, and photosynthetic efficiency). These results provide confidence for previous reports of this disease based upon visual assessments only, contribute to the development of monitoring and conservation strategies for safeguarding P. comosa forests, and generate insights into potential factors influencing host-pathogen interactions in this system.

10/05/2024


Hyoscyamine (HYO) and scopolamine (SCO) are tropane alkaloids acting as anticholinergic factors on the parasympathetic nervous system in humans and are produced by Solanaceous plants. Two strains of Agrobacterium rhizogenes, A4 and LBA9402, were used to infect Atropa acuminata Royle ex Miers and Atropa belladonna L. leaf explants. A. acuminata was inoculated either by direct infection or sonicated-assisted A. rhizogenes-mediated transformation (SAAT) was performed. A. belladonna was inoculated with the A4 strain using a direct method. The selected hairy root lines of both species were elicited with 50 mM methyl-β-cyclodextrin (β-CD), 0.5 μM coronatine (Cor) or 50 mM β-CD + 0.5 μM Cor on Day 14 of culture. The elicitor effect on growth and HYO and SCO content was analyzed after one (T1) and two (T2) weeks of treatment. In A. acuminata explants, the highest transformation percentage (T%) was obtained with strain A4 and the SAAT method (T% 96.43). Cor significantly reduced the growth of A. acuminata hairy roots (fresh weight and dry weight [DW] 2.52 and 0.3 g, respectively), whereas β-CD increased their DW (0.4 g). Also, the combined β-CD + Cor treatment had a positive significant effect on the DW of A. belladonna hairy roots (0.41 g). In A. acuminata hairy roots, the HYO level was lower under Cor treatment than in the control at both sampling times. In contrast, the SCO content was increased 10-fold by Cor elicitation at T1 compared to the control (10.95 mg g-1 DW) and was also positively affected by  β-CD + Cor. In A. belladonna hairy roots, all the elicitors had a negative effect on both HYO and SCO production. This report is the first assessment of the effect of β-CD and Cor elicitors on tropane alkaloid production.The extinction of species is a non-random process, and understanding why some species are more likely to go extinct than others is critical for conservation efforts. Functional trait-based approaches offer a promising tool to achieve this goal. In forests, deadwood-dependent (saproxylic) beetles comprise a major part of threatened species, but analyses of their extinction risk have been hindered by the availability of suitable morphological traits. To better understand the mechanisms underlying extinction in insects, we investigated the relationships between morphological features and the extinction risk of saproxylic beetles. Specifically, we hypothesised that species darker in colour, with a larger and rounder body, a lower mobility, lower sensory perception and more robust mandibles are at higher risk. We first developed a protocol for morphological trait measurements and present a database of 37 traits for 1,157 European saproxylic beetle species. Based on 13 selected, independent traits characterising aspidly growing field of ecological and conservation studies of deadwood.Camera traps are an increasingly popular tool to monitor wildlife distributions. However, traditional analytical approaches to camera trap data are difficult to apply to visible wildlife characteristics in single images, such as infection status. Several parasites produce visible signs of infection that could be sampled via camera traps. Sarcoptic mange Sarcoptes scabiei is an ideal disease to study using cameras because it results in visible hair loss and affects a broad host range. Here, we developed a multi-state occupancy model to estimate the occurrence of mange in coyotes Canis latrans across an urban gradient. This model incorporates a secondary detection function for apparent by-image infection status to provide detection-corrected estimates of mange occurrence. We analysed a multi-year camera trap dataset in Chicago, Illinois, United States, to test whether the apparent occurrence of sarcoptic mange in coyotes Canis latrans increases with urbanization or varies through time. We documented visible sigse. More generally, our model could provide detection-corrected occupancy estimates of visible characteristics that vary by image such as body condition or injuries.Gaslighting is an insidious, pernicious form of bullying that is gaining notoriety in nursing academia anecdotally. Attempting to identify the behaviors associated with gaslighting are difficult because of the subtle nature with which gaslighting is performed by the perpetrator. Traditional tools/models used for identifying bullying and horizontal violence are not sensitive enough to pick up gaslighting behaviors. Perpetual lies and mistruths, vexatious or frivolous complaints, praise and positive reinforcement, the use of blame or mobbing, and coercion are some of the tactics a gaslighter will use to create confusion and chaos for the gaslightee. The psychological harm for the gaslightee can lead to self-doubt, hypervigilance, depression, anxiety, addiction, and suicidal ideation as well as the physical symptoms associated with stress. Left with very few choices the gaslightee either adheres to the behavior or leaves. It is important to raise awareness and perhaps education and training of this form of bullying behavior because of the deleterious effects it has personally and professionally.Climate change may have dramatic consequences for communities through both direct effects of peak temperatures upon individual species and through interspecific mismatches in thermal sensitivities of interacting organisms which mediate changes in interspecific interactions (i.e. predation). Despite this, there is a paucity of information on the patterns of spatial physiological sensitivity of interacting species (at both landscape and local scales) which could ultimately influence geographical variation in the effects of climate change on community processes. In order to assess where these impacts may occur, we first need to evaluate the spatial heterogeneity in the degree of mismatch in thermal tolerances between interacting organisms. We quantify the magnitude of interspecific mismatch in maximum (CTmax ) and minimum (CTmin ) thermal tolerances among a predator-prey system of dragonfly and anuran larvae in tropical montane (242-3,631 m) and habitat (ponds and streams) gradients. To compare thermal mismatchey naiad predators may further increase the vulnerability of lowland tadpoles to warming due to potentially enhanced indirect effects of higher predation rates by more heat-tolerant dragonfly predators. However, further experimental work is required to establish the individual and population-level consequences of this thermal tolerance mismatch upon biotic interactions such as predator-prey. .
Microaggressions, a concept originally introduced for ethnic minorities, represent subtle day-to-day discrimination, damaging the psychological health and well-being of lesbian, gay, bisexual, and transgender individuals as well. This study aimed to assess whether microaggressions occur in psychotherapists' assessments of clients who identify as either lesbian or transgender woman when compared with those identifying as heterosexual woman.

The study included a sample of 135 licensed psychotherapists (110 cisgender women and 25 cisgender men). Participants were presented with an audio file of a woman introducing herself during her first therapy session. Three versions were presented a transgender, a lesbian, and a heterosexual client. Participants were asked to assess the clinical relevance of 10 questions defined as neutral (N = 5) and microaggressive (N = 5), used to determine a clinical impression of the client. A repeated measure analysis of variance was conducted to understand the likelihood of clients of different gender identity and sexual orientation receiving microaggressions.

Participants were more prone to consider microaggressive questions relevant where the client identified as either lesbian or transgender.

The findings highlighted a bias against lesbian and trans women in evaluating the relevance and usefulness of clinical information while making a psychological assessment of a case.
The findings highlighted a bias against lesbian and trans women in evaluating the relevance and usefulness of clinical information while making a psychological assessment of a case.Understanding the forces that shape species distributions is increasingly important in a fast-changing world. Although major disturbance events can adversely affect natural populations, they can also present new opportunities, for example by opening up habitat for colonization by other lineages. Following extensive geographic sampling, we use genomic data to infer a range extension following disturbance for an ecologically important intertidal macroalgal species. https://www.selleckchem.com/products/pci-34051.html Specifically, we genotyped 288 southern bull kelp (Durvillaea) plants from 28 localities across central New Zealand. All specimens from the North Island were expected to be D. antarctica, but unexpectedly 10 samples from four sites were identified as D. poha. Extensive sampling from the northern South Island (105 samples at five locations) confirmed the absence of D. poha north of the Kaikōura Peninsula. The North Island specimens of D. poha therefore reveal a biogeographic disjunction, some 150 km northeast of the nearest (South Island) population of this species. Based on strong geographic correspondence between these North Island samples and historic disturbance, we infer that tectonic upheaval, particularly earthquake-generated landslides, likely extirpated local D. antarctica and created an opportunity for a northward range expansion event by D. poha. Close phylogenomic relationships between this new North Island population and South Island samples support a geologically recent northward expansion, rather than a deeper evolutionary origin. These findings indicate the potential of large-scale disturbances to facilitate sudden biogeographic range expansions, and they emphasize the ability of genomic analyses with fine-scale sampling to reveal long-lasting signatures of past disturbance, dispersal, and colonization.Infectious diseases affecting habitat-forming species can have significant impacts on population dynamics and alter the structure and functioning of marine ecosystems. Recently, a fungal infection was described as the causative agent of necrotic lesions on the stipe of the forest-forming macroalga Phyllospora comosa, a disease named "stipe rot" (SR). Here, we developed a quantitative PCR (qPCR) method for rapid detection and quantification of this pathogen, which was applied to evaluate the level of SR infection in eight P. comosa populations spanning the entire latitudinal distribution of this species along southeastern Australia. We also investigated the relationship between the abundance and prevalence of Stipe Rot Fungus (SRF) and potential host chemical defenses as well as its relationship with morphological and ecophysiological traits of P. comosa. qPCR estimates of SRF abundance reflected the levels of infection estimated by visual assessment, with higher numbers of SRF copies being observed in individuals showing high or intermediate levels of visual symptoms of SR. Concordance of conventional PCR and visual assessments was 92 and 94%, respectively, compared to qPCR detection. SRF prevalence was positively related to fucoxanthin content and herbivory, but not significant related to other traits measured (phlorotannin content, total length, thallus diameter, stipe width, number of branches, frond width, fouling, bleaching, gender, and photosynthetic efficiency). These results provide confidence for previous reports of this disease based upon visual assessments only, contribute to the development of monitoring and conservation strategies for safeguarding P. comosa forests, and generate insights into potential factors influencing host-pathogen interactions in this system.

"In an excellent and fully referenced article aptly titled 'Let’s cut the crap on gene technology,' molecular biologist professor Jack Heinemann ... says society should be asking itself why it needs to trade the security of its GMO regulations for unsecured promises from the speculative visions of genetic engineers." — Claire Robinson, GMWatch

https://childrenshealthdefense.org/defender/gene-editing-myths-gaslighting-public/?utm_source=sovren&utm_medium=social&utm_campaign=defender&utm_id=20240923

In a referenced article, "Let's cut the crap on gene technology," molecular biologist Jack Heinemann says society should ask itself why it needs to trade the security of its GMO regulations for unsecured promises from the speculative visions of genetic engineers.

childrenshealthdefense.org