To understand the response of Calamagrostis angustifolia wetland of the Sanjiang Plain to changes in snow cover, we examined the greenhouse gases emission flux of the removed snow treatment (0 cm, RS), the added snow treatment (50 cm, AS) and the control (20 cm, CK) of a C. angustifolia wetland, and their relations with environmental factors with the method of the static chamber-gas chromatography. The results showed that soil temperature, soil water content, and carbon emissions were lowest during the snow-covering period under all treatments, and gradually increased with time. With the increases of time and snow thickness, soil temperature was rised and the difference of three treatments gradually was decreased. https://www.selleckchem.com/products/Pomalidomide(CC-4047).html Soil water content of RS was always lower than that of CK and AS. AS and CK could promote soil CO2 emission compared with RS during and after snowmelt. The soil cumulative CH4 emissions differed little among the treatments. There was significant correlation between soil temperature and cumulative CO2 and CH4 emissions. With the increases of soil temperature, soil cumulative CO2 emission continued to increase and soil cumulative CH4 emission decreased firstly and then increased rapidly. Soil water content was significantly correlated with cumulative CO2 and CH4 emissions. As the soil moisture increased, the cumulative soil CO2 emission gradually increased, reaching a certain threshold and then flattening, while soil cumulative CH4 emission continuously increased.A pot experiment with Huayu 25 as experimental material was conducted, with treatments of drought and salt stresses. The effects of drought and salt stresses at the flowering stage on the plant morphology, pod yield, and soil bacterial community structure in the rhizosphere were examined. The results showed that Proteobacteria, Actinobacteria, Saccharibacteria, Chloroflexi, Cyanobacteria, and Acidobacteria were the dominant phyla in the rhizosphere soil of peanut. Compared with that under normal conditions, the relative abundance of Proteobacteria and Actinobacteria dramatically decreased, while that of Cyanobacteria evidently increased in drought-treated and salt-treated soil. Moreover, the variation of Cyanobacteria abundance caused by combined drought and salt stresses was stronger than that caused by single drought or salt stress. Functional meta-genomic profiling indicated that a series of sequences related to signaling transduction, defense mechanism and post-translational modification, protein turnover, chaperones were enriched in rhizosphere soil under stressed conditions, which might have implications for plant survival and stress tolerance. Drought and salt stress affectedpeanut growth and reduced pod yield. Results from this study would present reference on the future improvement of stress tolerance of peanuts via modifying soil microbial community.Arbuscular mycorrhizal fungi (AMF) can promote nutrient absorption and improve stress resistance of host plants. The effects of AMF on aroma substance metabolism were rarely examined. In this study, we investigated the effects of AMF (Glomus mosseae) on glands and metabolism of aroma substances in tobacco leaves. The results showed that the density of gland hair and the relative expression of the glandular-specific lipid transporter gene NtLTP1, which was necessary to induce lipid secretion, were higher in the leaves of tobacco inoculated with AMF. The content of main aroma substances in tobacco leaves, such as carotenoids, chlorogenic acid and solanesol, were increased. Moreover, AMF inoculation increased the activities of phenylalanine ammonia-lyase (PAL) and polyphenol oxidase (PPO), the key aroma substance synthesis enzymes, and caused upregulation in the relative expression of phenylalanine transaminase, polyphenoloxidase, flavonoids alcoholase and squalene synthase encoding genes. In all, the symbiosis with G. mosseae could increase the abundance and secretory activity of glandular hairs and promote the synthesis of aroma substances in tobacco leaves.The basin region of nine highland lakes plays an important role in the economic development of Yunnan Province, in which, the basin region of the five lakes (Dianchi, Fuxianhu, Xingyunhu, Qiluhu and Yangzonghai Lake, hereinafter was called Five-lake Basin) locating in the central region of Yunnan Province is the most active economic and social development area of industry, agriculture and tourism, closely related to the eco-environmental quality of the whole basin. In this study, the eco-environmental quality of the Five-lake Basin from 1988 to 2018 was monitored and evaluated based on remote sensing ecological index (RSEI). The results showed that although the eco-environmental quality in this area had a trend of turning better from 1988 to 2018, with the mean value of RSEI increasing from 0.368 to 0.481, whereas the overall eco-environmental quality of the basin was poor. The area with improved eco-environmental quality accounted for 57.6%, which was mainly distributed in the mountains between lakes, while the declining area was mainly the flat area around the lake. The eco-environmental quality of Dianchi Lake, Fuxianhu Lake, and Yangzonghai Lake was improved during the study period, but the eco-environmental quality of Qiluhu Lake and Xingyunhu Lake deteriorated obviously in the last 10 years. The change of eco-environmental quality in each lake was consistent with the change of water quality. In the future, the improvement of eco-environmental quality needs more social attention and government investment.The Bailongjiang watershed of Gansu is an important water conservation and ecological barrier area in the upper reaches of Yangtze River. It is necessary to reveal the tradeoffs and synergies of ecosystem services (ESs) for the "win-win" of watershed ecological system and social eco-nomy development. Based on the InVEST model, four typical ESs including soil conservation (SC), water conservation (WC), food supply (FS), and habitat quality (HQ) were assessed, and the multi-scale tradeoffs and synergies of ESs and its drivers were analyzed by correlation and root mean square deviation (RMSD). The results showed that there were significant synergies among SC, WC, and HQ, and a significant tradeoff between FS and HQ, SC, WC, respectively. The areas with high tradeoff intensity between the three pairs of synergistic services (SC-WC, SC-HQ, WC-HQ), and between FS and HQ were mainly concentrated in the steep forest area of middle-high mountain in Wenxian, Diebu and Zhouqu. The high intensity of tradeoffs between FS-SC, FS-WC were mainly concentrated in the gentle apricus farming and pastoral areas of middle-low mountain in Tanchang and Wudu.

We did not detect an acute, short-lag PM2.5 effect nor short-lag temperature effect on influenza in Montana. Higher daily average PM2.5 concentrations during the wildfire season was positively associated with increased influenza in the following winter influenza season (expected 16% or 22% increase in influenza rate per 1 μg/m3 increase in average daily summer PM2.5 based on two analyses, p = 0.04 or 0.008). This is one of the first observations of a relationship between PM2.5 during wildfire season and influenza months later. Human immunodeficiency virus type 1 (HIV-1) is a public health problem that affects over 38 million people worldwide. Although there are highly active antiretroviral therapies, emergence of antiviral resistant strains is a problem which leads to almost a million death annually. Thus, the development of new drugs is necessary. The viral enzyme reverse transcriptase (RT) represents a validated therapeutic target. Because the oxoquinolinic scaffold has substantial biological activities, including antiretroviral, a new series of 4-oxoquinoline ribonucleoside derivatives obtained by molecular hybridization were studied here. All synthesized compounds were tested against human immunodeficiency virus type 1 reverse transcriptase (HIV-1 RT), and 9a and 9d displayed the highest antiviral activities, with IC50 values of 1.4 and 1.6 μM, respectively. These compounds were less cytotoxic than AZT and showed CC50 values of 1486 and 1394 μM, respectively. Molecular docking studies showed that the most active compounds bound to the allosteric site of the enzyme, suggesting a low susceptibility to the development of antiviral resistance. In silico pharmacokinetic and toxicological evaluations reinforced the potential of the active compounds as anti-HIV candidates for further exploration. Overall, this work showed that compounds 9a and 9d are promising scaffold for future anti-HIV-1 RT drug design. Due to the increasing resistance of Pseudomonas aeruginosa to most clinically relevant antimicrobials, it is challenging to treat bacterial infection with traditional antibiotics. Quorum sensing can regulate the production of biofilms and virulence factors which are closely related to bacterial resistance. Previously we synthesized a series of oxazolidinone compounds targeting the quorum-sensing transcriptional regulatory protein CviR and ZS-12 showed good activity against Chromobacterium violaceum CV026 quorum-sensing. In this study, eighteen 3-amino-2-oxazolidinone compounds were designed and synthesized using ZS-12 as the lead compound. We initially evaluated the inhibitory activities of novel oxazolidinone compounds against QS using C. violaceum CV026 as a reporter strain. Thirteen compounds showed good activities (IC50 range 3.69-63.58 μM) and YXL-13 inhibition was the most significant (IC50 = 3.686 ± 0.5790 μM) against biofilm formation and virulence factors determination of P. aeruginosa PAO1. In vitro, YXL-13 significantly inhibited the formation of PAO1 biofilm (range 42.98%-17.67%), the production of virulence factors (pyocyanin, elastase, rhamnolipid, and protease), and bacterial motility. Moreover, the combination of YXL-13 with an antibiotic (meropenem trihydrate) could significantly improve the antibiotic susceptibility of biofilm P. aeruginosa PAO1 cells. In vivo, YXL-13 significantly prolonged the lifespan of wildtype Caenorhabditis elegans N2 infected by P. aeruginosa PAO1. In conclusion, YXL-13 is a candidate agent for antibiotic-resistant P. aeruginosa PAO1and provides a method for finding new antibacterial drugs. Lipoic acid (LA) and its reduced form (dihydrolipoic acid, DHLA) have unique antioxidant properties among such molecules. Moreover, after a process termed lipoylation, LA is an essential prosthetic group covalently-attached to several key multi-subunit enzymatic complexes involved in primary metabolism, including E2 subunits of pyruvate dehydrogenase (PDH). The metabolic pathway of lipoylation has been extensively studied in Escherichia coli and Arabidopsis thaliana in which protein modification occurs via two routes de novo synthesis and salvage. Common to both pathways, lipoyl synthase (LIP1 in plants, LipA in bacteria, EC 2.8.1.8) inserts sulphur atoms into the molecule in a final, activating step. However, despite the detection of LA and DHLA in other plant species, including tomato (Solanum lycopersicum), no plant LIP1s have been characterised to date from species other than Arabidopsis. In this work, we present the identification and characterisation of two LIPs from tomato, SlLIP1 and SlLIP1p. Consistent with in silico data, both are widely-expressed, particularly in reproductive organs. In line with bioinformatic predictions, we determine that yellow fluorescent protein tagged versions of SlLIP1 and SlLIP1p are mitochondrially- and plastidially-localised, respectively. Both possess the molecular hallmarks and domains of well-characterised bacterial LipAs. When heterologously-expressed in an E. coli lipA mutant, both are capable of complementing specific growth phenotypes and increasing lipoylation levels of E2 subunits of PDH in vivo, demonstrating that they do indeed function as lipoyl synthases. Symbiotic plant-microorganisms interaction is a promising approach to avoid the environmental hazards of synthetic fertilizers and pesticides. Among these, arbuscular mycorrhizal fungi (AMF) are known to improve the growth and quality of many plant species; however the detailed metabolic mechanisms behind such beneficial effects are far from complete. Further, elevated levels of atmospheric CO2 (eCO2) could affect such AMF-plant association. https://www.selleckchem.com/products/Sodium-valproate.html Herein, we have investigated the individual and synchronous impact of AMF and eCO2 (620 ppm) on nutrient uptake, growth, photosynthesis, respiration, and levels of primary and secondary metabolites in oregano (Oreganum vulgare), an economically important herbal plant. Enhanced AMF colonization rate and a better mycelial growth were observed in roots of oregano grown under eCO2. Both AMF and eCO2 treatments significantly enhanced the growth and photosynthesis of oregano plants, however much improvements were observed by their synchronous application. eCO2 further increased the AMF-induced dark respiration and accumulation of macro and microelements.

© 2020 Published by Elsevier Ltd.Manganese, zinc, and iron would be the most crucial micronutrients required for plant development and applied as foliar fertilizers. Herein, a simple template-free microwave-assisted hydrothermal green synthesis technique was adjusted to create manganese zinc ferrite nanoparticles (Mn0.5Zn0.5Fe2O4 NPs) at different temperatures (100, 120, 140, 160 and 180 °C). The prepared nanomaterials had been used at different levels (0, 10, 20, and 30 ppm) as foliar nanofertilizers throughout the squash (Cucurbita pepo L) planting process. X-ray diffraction patterns associated with the prepared nanomaterials verified successful production associated with the nanoferrite material. The prepared nanofertilizers revealed type IV adsorption isotherm feature for mesoporous products. FE-SEM and HR-TEM imaging showed that the nanoparticles had been cubic shaped and increased in particle size utilizing the boost in microwave oven temperature during production. The impact of application of this synthesized ferrite nanoparticles on vegetative growth, proximate analysthor(s).In this study, livestock herders in eastern Sudan had been interviewed through organized survey included 14046 pets in 151 herds (87 camel herds, 51 sheep and 13 goats) from Summer to September of 2016 in Showak part of Gadarif State to have some epidemiological informative data on infectious ecthyma (CE) disease. 102 suspected situations of CE had been investigated (38 sheep, 22 goats and 42 camels) by a moment survey focusing on age and sex of affected animals beside number and localization of the lesions. Representative tissue types of scab lesion scrapings had been collected from a complete of 36 suspected sheep, goats and camels for DNA removal to identify PPV by quantitative real-time PCR and gel-based PCR, then a PCR protocol had been used to have DNA fragment of B2L gene from six DNAs (2 from each animal species) for sequencing. Phylogenetic tree predicated on nucleotide sequences had been constructed and all data had been analyzed statistically. Obtained result shows morbidity price of 23.8% and a case fatality rate near to the Pseudocowpoxvirus (PCPV) types of the PPV genus and share 92.51-93.62 percent identity with the GPPV isolates. In conclusion the current study demonstrated that the gross lesion made by PPV in sheep, goats and camels is usually similar https://catalasesignaling.com/index.php/blended-one-respiratory-as-well-as-hard-working-liver-hair-transplant/ , yet the PPVs circulating in eastern Sudan in camels (PCPV) are genetically distinct from those affecting sheep and goats (ORFV). Infectious ecthyma in east Sudan triggers significant morbidities and mortalities and control steps, directed by the results of this research should really be implemented. © 2020 The Author(s).A bench-scale model of a partially packed upflow anaerobic fixed film (UAF) reactor was put up and operated at five different hydraulic retention times (HRTs) of (17, 14, 10, 8, and 5) days. The reactor was provided with artificial rubberized wastewater comprising a chemical oxygen need (COD) concentration of 6355-6735 mg/L. The results had been examined utilizing the Monod design, the changed Stover-Kincannon models, therefore the Grau Second-Order Model. The Grau Second-Order model was discovered to best fit the experimental information. The biokinetic continual values, specifically the development yield coefficient (Y) in addition to endogenous coefficient (Kd) were 0.027 g VSS/g COD and 0.1705 d-1, correspondingly. The half-saturation constant (Ks) and maximum substrate usage rate (K) came back values of 84.1 mg/L and 0.371 d-1, correspondingly, whereas the most particular growth price of the microorganism (μmax) ended up being 0.011 d-1. The constants, Umax and KB, of the Stover-Kincannon design produced values of 6.57 g/L/d and 6.31 g/L/d, correspondingly. Meanwhile, the typical second-order substrate removal rate, ks(2), had been 105 d-1. These models gave high correlation coefficients because of the value of R2 = 80-99% and these suggested that these models can be utilized in creating UAF reactor consequently predicting the behaviour for the reactor. © 2020 The Authors.Toxic pyrrolizidine alkaloids (PAs) and their N-oxides (PANOs) is present in bee pollen with regards to the flowers seen by bees. A liquid chromatography-mass spectrometry (LC-MS/MS) method was developed and validated to monitor 17 PAs/PANOs in 44 bee pollens. The CIE-L∗a∗b∗ colour coordinates with all the specular element either included or omitted were taped in pellets and ground aliquots. Lightness (L∗) and yellowness (b∗) of surface bee pollen had been significantly correlated to PAs/PANOs content. The L∗ and b∗ cut-offs sorted by a receiver operating characteristic analysis to anticipate PAs/PANOs existence showed an important upsurge in the relative threat to detect quantities greater than 84 μg kg-1. Two supervised canonical discriminant analyses confirmed that pollen without PAs might be distinguished from those containing PAs/PANOs. The info declare that instrumental color in conjunction with supervised designs could be used as a screening test for PAs/PANOs in bee pollen, before the confirmatory LC-MS/MS analysis. © 2020 The Author(s).In the current research, altered removal practices utilizing supercritical CO2 were investigated so that you can get high-added price substances from rapeseed oil deodorizer distillate and reviews had been done with changed Soxhlet extraction (solvent extraction + silica). For supercritical substance extraction (SFE), the suitable extraction parameters were temperature of 40 °C, force of 350 bar (for phytosterols), 400 bar (for tocopherol), 5 wt% ethanol as co-solvent, and saponification pretreatment. The optimized SFE process led to the recovery of three main phytosterols (50 wt % β-sitosterol, 23.91 wt percent Brassicasterol, and 36.25 wt % Campesterol) and only α-tocopherol. More over, there is no synergistic result with saponification pretreatment + co-solvent while the performance and concentration of target compounds had been lower than supercritical CO2 + co-solvent. Additionally, comparative Data showed that the effectiveness of phytosterols and tocopherols had been roughly three times greater (p less then 0.05) in SFE relative to modified Soxhlet extraction.

The framework first addresses theoretical potential and successively constrains this further by stepwise inclusion of technical, economical, and sustainability criteria to obtain the sustainable exploitable hydropower potential. We conclude that sustainable hydropower potential in complex basins such as the Indus goes far beyond the hydrological boundary conditions. Our framework enables the careful inclusion of factors beyond the status-quo technological and economic criterions to guide policymakers in hydropower development decisions in the Indus and beyond. Future work will implement the framework to quantify the different hydropower potential classes and explore adaptation pathways to balance SDG7 with the other interlinked SDGs in the Indus.To address the CO2 emissions issue, China promised to increase its nationally determined contributions, trying to reach a CO2 emissions peak by 2030. For optimizing emission reduction policies, it is important to clarify the CO2 linkage structure and transfer characteristics. Previous research mainly focused on the calculation and comparison of CO2 linkage at the national level or the regional level and lacked inter-provincial sector-sector transfer analysis. This study uses hypothetical extraction method (HEM) to calculate the inter-provincial sectoral linkages of embodied CO2 in 2012 and 2015, providing a new perspective for sectoral CO2 linkage studies in China. We use net transfer to reveal the impact of provincial trade on the embodied CO2 emissions, and identify key CO2 emitter sectors. Combined with complex networks, we describe the clustering feature visualized and identify the transfer media sectors. The results are as follows (1) the key sectors with large linkage are mainly the heavy industries located in North China. The electricity industry has the largest net CO2 outflow as the energy supplier, whereas the construction industry has the largest net inflow as the driving sector. https://www.selleckchem.com/products/ad-5584.html (2) The CO2 transfer networks present closely connected and spatial clustering features, reflecting the embodied CO2 linkage between geographically adjacent sectors closer. (3) The important media sectors are mostly located in northwest China with small industrial scale and linkage degrees, such as the transport equipment industry in Shanxi. Emission reduction policies should be overall planned and tailored to local conditions. Consequently, possible policy implications of the results are discussed, which could provide additional insights for CO2 mitigation.Research evidence suggests that pesticide residues are one of the leading potential causes of the decline in pollinators, especially during vulnerable periods such as foraging in the early springtime. In China, no research quantifies pesticide residues in the nectar and pollen of honey bee colonies during this period or examines the potential risks and toxicity of pesticides to honey bees. Oilseed rape is one of the first and primary bee-attractive plants in most parts of China. Here, we investigated the pesticide residues in the oilseed rape of the years 2017 and 2018 in China. The hazard quotient (HQ) from pollen and nectar and the BeeREX risk assessment were used to evaluate the potential risks of the pesticide residues to honey bees. We detected 48 pesticides in pollen samples and 34 chemicals in nectar samples. The maximum pollen HQ (PHQ) values (contact or oral) ranged from 0.16 to 706,421, and the maximum nectar HQ (NHQ) values (contact or oral) ranged from 0.07 to 185,135. In particular, carbofuran, cyfluthrin, deltamethrin, and fenpropathrin have relatively high PHQ and NHQ values. Our results indicated that further investigation of nearly half of the tested compounds is needed because their PHQ or NHQ values are more than 50. Especially cyfluthrin and carbofuran need advanced tier assessment due to their maximum RQ (risk quotient) values exceeding the level of concern. These results provide valuable guidance for protecting bees and other pollinators in China.Dynamic transfer of radionuclides to fish was studied in a series of experiments under field condition in two lakes within the Chernobyl exclusion zone during 2016-2020. "Clean" common rudd (Scardinius erythrophthalmus) and silver Prussian carp (Carassius gibelio) were transported to the contaminated Glubokoye Lake and kept in cages during several months of exposure, while contaminated Glubokoye fish were kept in cages in the "clean" Starukha Lake. Radiocaesium (137Cs) and radiostrontium (90Sr) were determined in intestine contents, muscle and bone tissues based on repeated samples during several months of exposure. During summer, the activity concentrations of 137Cs and 90Sr increased with time of exposure in clean fish caged in the contaminated lake. During autumn and winter, however, minor changes in fish uptake occurred during several weeks of exposure to the contaminated water. Furthermore, depuration in the contaminated fish was significant during summer, while insignificant during winter when exposed in the «clean» water. The rate constant of 137Cs uptake in muscle was between 8.0 and 22 day-1 during summer, while 0.2 to 1.0 day-1 during autumn-winter. Similarly, the rate constant of 90Sr uptake in bone was between 1.4 and 1.6 day-1, while 0.08-0.52 day-1 during autumn-winter. Biological half-lives of 137Cs in fish muscle tissue in summer were 77 ± 10 days, while exceeded 230 days during seasons at low water temperature. The results demonstrated that the transfer of 137Cs and 90Sr to fish was highly dependent upon seasons, in particular the water temperature. The transfer data obtained during low water temperature seasons deviated significantly from transfer data in literature and handbooks. Thus, seasonal changes in radionuclide transfer to fish should be taken into account when radiological impact to fish is assessed.The knowledge regarding the spatial ecology of red deer (Cervus elaphus) in different environments is crucial if effective management actions are to be designed. However, this knowledge continues to be scarce in the complex contexts of mixed land use and management circumstances. This study describes the spatial ecology of red deer monitored using GPS collars in Mediterranean ecosystems of South-Central Spain, considering the effect of individual and seasonal (food shortage period, rut, hunting season and food abundance period) factors on different land use and management scenarios, namely protected areas, mixed farms and fenced hunting estates. Our results showed less activity (ACT), a shorter daily range (DR) and a smaller home range (HR) during the food shortage period ACT 0.38 ± (SD) 0.12; DR 3010.9 ± 727.3 m; and weekly HR 122.2 ± 59.6 ha. With regard to land use, individuals were less ACT and had a smaller DR on fenced hunting estates (ACT 0.24 ± 0.12; DR 1946.3 ± 706.7 m) than in protected areas (ACT 0.

Ligand-induced protein degradation has emerged as a compelling approach to promote the targeted elimination of proteins from cells by directing these proteins to the ubiquitin-proteasome machinery. So far, only a limited number of E3 ligases have been found to support ligand-induced protein degradation, reflecting a dearth of E3-binding compounds for proteolysis-targeting chimera (PROTAC) design. Here, we describe a functional screening strategy performed with a focused library of candidate electrophilic PROTACs to discover bifunctional compounds that degrade proteins in human cells by covalently engaging E3 ligases. Mechanistic studies revealed that the electrophilic PROTACs act through modifying specific cysteines in DCAF11, a poorly characterized E3 ligase substrate adaptor. We further show that DCAF11-directed electrophilic PROTACs can degrade multiple endogenous proteins, including FBKP12 and the androgen receptor, in human prostate cancer cells. Our findings designate DCAF11 as an E3 ligase capable of supporting ligand-induced protein degradation via electrophilic PROTACs.α-MnO2 nanorods and flower-like γ-MnO2 microspheres were synthesized by facile and mild methods to illustrate the effect of crystal structures and surface features on catalytic performance with the help of carbon monoxide (CO) oxidation. It is revealed that the flower-like γ-MnO2 microspheres possess better catalytic oxidation performance (CO complete conversion temperature at 120 °C and long-time stability for 50 h) than α-MnO2 nanorods, which can be attributed to the obvious differences in the chemical bonds and linking modes of [MnO6] octahedra due to the different crystal structures. https://www.selleckchem.com/products/VX-770.html γ-MnO2 possesses lower Mn-O bond strength that enables γ-MnO2 to present a large amount of surface lattice oxygen and superior oxygen mobility. The disordered random intergrowth tunnel structure can adsorb effectively CO molecules, resulting in excellent catalytic performance for CO catalytic oxidation. In addition, the MnO2 catalyst probably occurred via a Mars-van Krevelen mechanism for CO oxidation. This work provides an insight into the effect of crystal structures and surface property of manganese oxide on catalytic oxidation performance, which presents help for the future design of promising catalysts with excellent catalytic performance.Significant advances have been made in the synthesis of chemically selective environments within metal-organic frameworks, yet materials development and industrial implementation have been hindered by the inability to predictively control crystallite size and shape. One common strategy to control crystal growth is the inclusion of coordination modulators, which are molecular species designed to compete with the linker for metal coordination during synthesis. However, these modulators can simultaneously alter the pH of the reaction solution, an effect that can also significantly influence crystal morphology. Herein, noncoordinating buffers are used to independently control reaction pH during metal-organic framework synthesis, enabling direct interrogation of the role of the coordinating species on crystal growth. We demonstrate the efficacy of this strategy in the synthesis of low-dispersity single-crystals of the framework Co2(dobdc) (dobdc4-= 2,5-dioxido-1,4-benzenedicarboxylate) in a pH 7-buffered solution using cobalt(II) acetate as the metal source. Density functional theory calculations reveal that acetate competitively binds to Co during crystallization, and by using a series of cobalt(II) salts with carboxylate anions of varying coordination strength, it is possible to control crystal growth along the c-direction. Finally, we use zero length column chromatography to show that crystal morphology has a direct impact on guest diffusional path length for the industrially important hydrocarbon m-xylene. Together, these results provide molecular-level insight into the use of modulators in governing crystallite morphology and a powerful strategy for the control of molecular diffusion rates within metal-organic frameworks.The first catalytic intermolecular 1,2-alkylborylation reaction via a radical-relay mechanism between unactivated olefins, bis(pinacolato)diboron, and an alkyl electrophile is reported. Successful implementation of this method requires that the competing boryl substitution of the alkyl electrophile is retarded to facilitate the radical relay. This challenge was overcome using electronically or sterically demanding alkyl electrophiles, which results in the simultaneous and highly regioselective introduction of a gem-difluoro, monofluoro, tertiary, or secondary alkyl group and a boryl group across the C═C double bond.Inorganic nanostructured materials such as silicon, carbon, metals, and metal oxides have been explored as matrices of low-background signals to assist the laser desorption/ionization (LDI) mass spectrometric (MS) analysis of small molecules, but their applications for imaging of small molecules in biological tissues remain limited in the literature. Titanium dioxide is one of the known nanoparticles (NP) that can effectively assist LDI MS imaging of low molecular weight molecules (LMWM). TiO2 NP is commercially available as dispersions, which can be applied using a chemical solution sprayer. However, aggregation of NP can occur in the dispersions, and the aggregated NP can slowly clog the sprayer nozzle. In this work, the use of zinc oxide (ZnO) NP for LDI MS imaging is investigated as a superior alternative due to its dissolution in acidic pH. ZnO NP was found to deliver similar or better results in the imaging of LMWM in comparison to TiO2 NP. The regular acid washes were effective in minimizing clogging and maintaining high reproducibility. High-quality images of mouse sagittal and rat coronal tissue sections were obtained. Ions were detected predominately as Na+ or K+ adducts in the positive ion mode. The number of LMWM detected with ZnO NP was similar to that obtained with TiO2 NP, and only a small degree of specificity was observed.Multidimensional NOESY experiments targeting correlations between exchangeable imino and amino protons provide valuable information about base pairing in nucleic acids. It has been recently shown that the sensitivity of homonuclear correlations involving RNA's labile imino protons can be significantly enhanced, by exploiting the repolarization brought about by solvent exchanges. Homonuclear correlations, however, are of limited spectral resolution, and usually incapable of tackling relatively large homopolymers with repeating structures like RNAs. This study presents a heteronuclear-resolved version of those NOESY experiments, in which magnetization transfers between the aqueous solvent and the nucleic acid protons are controlled by selecting specific chemical shift combinations of a coupled 1H-15N spin pair. This selective control effectively leads to a pseudo-3D version of HSQC-NOESY, but with cross-peaks enhanced by ∼2-5× as compared with conventional 2D NOESY counterparts. The enhanced signal sensitivity as well as access to both 15N-1H and 1H-1H NOESY dimensions can greatly facilitate RNA assignments and secondary structure determinations, as demonstrated here with the analysis of genome fragments derived from the SARS-CoV-2 virus.