icine. All rights reserved. For permissions, please e-mail journals.permissions@oup.com.The disturbed integrity of myelin and white matter, along with dysregulation of the lipid metabolism, may be involved in schizophrenia pathophysiology. Considering the crucial role of sphingolipids in neurodevelopment, particularly in oligodendrocyte differentiation and myelination, we examined the role of sphingolipid dynamics in the pathophysiology of schizophrenia. We performed targeted mass spectrometry-based analysis of sphingolipids from the cortical area and corpus callosum of postmortem brain samples from patients with schizophrenia and controls. We observed lower sphingosine-1-phosphate (S1P) levels, specifically in the corpus callosum of patients with schizophrenia, but not in major depressive disorder or bipolar disorder, when compared with the controls. Patient data and animal studies showed that antipsychotic intake did not contribute to the lowered S1P levels. We also found that lowered S1P levels in the corpus callosum of patients with schizophrenia may stem from the upregulation of genes for S1P-degrading enzymes; higher expression of genes for S1P receptors suggested a potential compensatory mechanism for the lowered S1P levels. A higher ratio of the sum of sphingosine and ceramide to S1P, which can induce apoptosis and cell-cycle arrest, was also observed in the samples of patients with schizophrenia than in controls. These results suggest that an altered S1P metabolism may underlie the deficits in oligodendrocyte differentiation and myelin formation, leading to the structural and molecular abnormalities of white matter reported in schizophrenia. Our findings may pave the way toward a novel therapeutic strategy. © The Author(s) 2020. Published by Oxford University Press on behalf of the Maryland Psychiatric Research Center.AIMS Human influenza A virus (hIAV) infection is associated with important cardiovascular complications, although cardiac infection pathophysiology is poorly understood. We aimed to study the ability of hIAV of different pathogenicity to infect the mouse heart, and establish the relationship between the infective capacity and the associated in vivo, cellular and molecular alterations. METHODS AND RESULTS We evaluated lung and heart viral titers in mice infected with either one of several hIAV strains inoculated intranasally. Three-dimensional reconstructions of infected cardiac tissue were used to identify viral proteins inside mouse cardiomyocytes, Purkinje cells, and cardiac vessels. Viral replication was measured in mouse cultured cardiomyocytes. Human induced pluripotent stem cells-derived cardiomyocytes (hiPSC-CMs) were used to confirm infection and study underlying molecular alterations associated with the in vivo electrophysiological phenotype. Pathogenic and attenuated hIAV strains infected and replicSociety of Cardiology.The fall armyworm Spodoptera frugiperda (J. E. Smith) invaded several West African countries in 2016 causing severe injury to maize plants and economic damage. This study assesses variations in the occurrence of this species in different Agro-Ecological Zones (AEZs) in Togo and Ghana during the 3 yr following its discovery. The surveys were conducted on 120 farms in Togo and 94 farms in Ghana by collecting larvae from 200 maize plants per hectare. Infestation levels were 68.46% in 2016, 55.82% in 2017, and 17.76% in 2018. The number of larvae recorded per hectare and infestation levels were higher in Togo than in Ghana. The lowest number of collected larvae and infestation levels of S. frugiperda were in 2018, compared to the other 2 yr. Larvae per hectare and the infestation level varied regionally inside the two countries. The southern part of Togo (AEZ five) contained higher numbers of larvae and higher infestation levels during the 2 yr following the invasion of the pest. We concluded that infestation levels of S. frugiperda are much lower in 2018 than the two previous years and it is therefore necessary to determine the factors that affect the population dynamics of S. frugiperda in the field, which is a perquisite for developing management interventions. © Published by Oxford University Press on behalf of Entomological Society of America 2020.OBJECTIVE To simulate the mechanical and fracture behaviors of cancellous bone in three anatomical directions and to develop an equivalent constitutive model. METHOD Micro-scale XFEM models of a cancellous specimen were developed with mechanical behaviors in three anatomical directions. An appropriate ABAQUS macro-scale model replicated the behavior observed in the micro-scale models. The parameters were defined based on the intermediate bone material properties in the anatomical directions and assigned to an equivalent non-porous specimen of the same size. The equivalent model capability was analyzed by comparing the micro- and macro- models. RESULTS The hysteresis graphs of the micro-scale model show that the modulus is the same in loading and unloading; similar to the metal plasticity models. The strength and failure strains in each anatomical direction are higher in compression than in tension. The micro-scale models exhibited an orthotropic behavior. Appropriate parameters of cast iron plasticity model were chosen to generate macro-scale models that are capable of replicating the observed micro-scale behavior of cancellous bone. CONCLUSION Cancellous bone is an orthotropic material that can be simulated using a cast iron plasticity model. This model is capable of replicating the micro-scale behavior in FE analysis simulations without the need for individual trabecula, leading to a reduction in computational resources without sacrificing model accuracy. Also, XFEM of cancellous bone compared to traditional FEM proves to be a valuable tool to predict and model the fractures in the bone specimen. Copyright (c) 2020 by ASME.In recent years, the OpenNotes movement and other changes in healthcare have driven institutions to make medical records increasingly transparent. As patients have begun to question and request changes to their Problem Lists, clinicians have come to face the ever more frequent challenge of discerning which changes to make and which to refuse. Now clinicians and patients together choose the list of problems that represent the patient's current state of health and illness. As the physician's role slides closer to consultant and the medical paternalism of the twentieth century falls further into the background of our technology-infused present, who holds the power of delineating a patient's clinical identity? This paper examines the ethical and practical dimensions of this question and proposes a research agenda that aims to answer it. Such explorations are essential to ensuring that the physician remains relevant to patient's notions of health, illness, intervention, and healing. © The Author(s) 2020. Published by Oxford University Press on behalf of the American Medical Informatics Association. All rights reserved. For permissions, please email journals.permissions@oup.com.During the migration of cancer cells for metastasis, cancer cells can be exposed to fluid shear conditions. We examined two breast cancer cell lines, MDA-MB-468 (less metastatic) and MDA-MB-231 (more metastatic), and a benign MCF-10A epithelial cell line for their responsiveness in migration to fluid shear. We tested fluid shear at 15 dyne/cm2 that can be encountered during breast cancer cells travelling through blood vessels or metastasizing to mechanically active tissues such as bone. MCF-10A exhibited the least migration with a trend of migrating in the flow direction. Intriguingly, fluid shear played a potent role as a trigger for MDA-MB-231 cell migration, inducing directional migration along the flow with significantly increased displacement length and migration speed and decreased arrest coefficient relative to unflowed MDA-MB-231. In contrast, MDA-MB-468 cells were markedly less migratory than MDA-MB-231 cells, and responded very poorly to fluid shear. As a result, MDA-MB-468 cells did not exhibit noticeable difference in migration between static and flow conditions, as was distinct in root mean square (RMS) displacement - an ensemble average of all participating cells. These may suggest that the difference between more metastatic MDA-MB-231 and less metastatic MDA-MB-468 breast cancer cells could be at least partly involved with their differential responsiveness to fluid shear stimulatory cues. Our study provides new data in regard to potential crosstalk between fluid shear and metastatic potential in mediating breast cancer cell migration. Copyright (c) 2020 by ASME.OBJECTIVES To compare the short-term outcomes of modified SEC III protocol in growing patients in relation to their compliance with the chincup, matching them with an untreated Class III control group (CG). MATERIALS AND METHODS Thirty-four patients (mean age of 8.6 ± 1.2 years) with Class III dentoskeletal malocclusion treated with the modified SEC III protocol and divided into group 1 (G1, 18 subjects), reporting good compliance and group 2 (G2, 16 subjects) not compliant with the chincup. The records were analysed before treatment (T1) and after the orthopaedic phase (T2), with an interval period of about 1.3 ± 0.5 years. The CG consisted of 16 untreated subjects with Class III dentoskeletal malocclusion (mean age of 8.3 ± 1.4 years). The statistical comparisons among the three groups were performed with ANOVA with Tukey's post hoc tests. RESULTS Both G1 and G2, compared with CG, presented significant improvements in the sagittal skeletal relationships (ANB +2.5° and +2.5°, Wits +4.2° and +3.0°, respectively). G1 exhibited a significant more favourable control of vertical skeletal relationships when compared to G2 (Palatal Pl.-Mandibular Pl. -2.0°) that were associated with a statistically significant reduction of the gonial angle (ArGoMe -1.7°). https://www.selleckchem.com/products/SNS-032.html Limitations of the study were its retrospective nature and the lack of evaluation of the long-term outcomes. CONCLUSIONS Both groups of treated patients showed favourable sagittal skeletal improvement compared with controls. The use of chincup in patients with good compliance produced significantly greater control of the skeletal vertical dimensions compared with patients not compliant with the chincup. © The Author(s) 2020. Published by Oxford University Press on behalf of the European Orthodontic Society. All rights reserved. For permissions, please email journals.permissions@oup.com.P values and error bars help readers infer whether a reported difference would likely recur, with the sample size n used for statistical tests representing biological replicates, independent measurements of the population from separate experiments. We provide examples and practical tutorials for creating figures that communicate both the cell-level variability and the experimental reproducibility. © 2020 Lord et al.Falling backward can lead to injuries including hip fracture, back injury, and traumatic brain impact among older adults. A loss of consciousness is associated with falling backward and accounts for about 13% of all falls among older adults. Little is known about the dynamics of backward falls, such as the falling duration, the impact severity, and how the fall dynamics are affected by the biomechanical properties of the lower limb joints, particularly the rotational stiffness. The purpose of this study was to investigate the influence of the stiffness of individual leg joints on the dynamics of backward falls after losing consciousness in terms of the falling duration and impact velocities. Based on a 15-segment human model, we simulated the process of falling backwards by sweeping the parameter space of ankle, knee, and hip's stiffness varying from 0 to 8.73 Nm/deg (or 500 Nm/rad). The results revealed that the falling duration and impact speeds of the head and hip ranged from 0.27 to 0.63 s, 2.65 to 7.88 m/s, and 0.