On the other hand, laminin caused cytoplasmic retention of YAP in IPAH PAEC. Accordingly, silencing of COL4A5 and LAMC1, respectively, differentially affected tight junction formation and barrier integrity in both donor and IPAH PAEC. Collectively, our results highlight the importance for a well-maintained BM homeostasis. By linking changes in BM structure and composition to altered endothelial cell function, we here suggest an active involvement of the BM in IPAH pathogenesis.Background Mortal obligate RNA transcript (MORT), a long noncoding RNA, has been reported as a potential tumor suppressor in many types of cancer. The functions of MORT involved in lung adenocarcinoma (LUAD) were investigated in this study. Materials and Methods A total of 67 patients with LUAD (adenocarcinoma) were recruited in this study. https://www.selleckchem.com/products/SP600125.html Quantitative reverse transcription-polymerase chain reaction was used to assess gene expression. Cell transfections were used to analyze gene interactions. Transwell migration and invasion assay were carried out to analyze cell migration and invasion. Results MORT was downregulated, whereas miRNA-223 was upregulated in LUAD. Expression of MORT was significantly affected by tumor metastasis but not by the size of tumors. Expression of miRNA-223 and MORT was inversely correlated in LUAD tissue samples. LUAD cells overexpressing MORT showed downregulated miRNA-223, whereas the expression of MORT was not significantly affected by overexpression of miRNA-223. Besides, overexpression of MORT inhibited, whereas overexpression of miRNA-223 promoted the invasion and migration of LUAD cells. Overexpression of miRNA-223 inhibited the effects of overexpressing MORT on cell invasion and migration. Conclusions Therefore, MORT may inhibit cancer cell invasion and migration in LUAD by downregulating miRNA-223.PURPOSE To evaluate the feasibility of brigade-style, multiphasic cancer screening in Honduras, exploring data from 3 screening events that each tested for multiple cancers on single occasions. METHODS This series of 3 studies each used a single-arm, post-test-only design to explore the feasibility of implementing multiphasic, community-based cancer screening at the same rural location in 2013, 2016, and 2017. The 2013 event for women screened for 2 cancers (breast and cervix), and the 2016 event for women screened for 3 cancers (breast, cervix, and thyroid). The 2017 event for men screened for 5 cancers (skin, prostate, colorectal, oropharynx, and testes). RESULTS Totals of 473 and 401 women participated in the 2013 and 2016 events, respectively, and 301 men participated in the 2017 event. Staffing for each event varied from 33 to 44 people and relied primarily on in-country medical students and local community members. High rates (mean, 88%) of compliance with referral for follow-up testing at clinics and primary care facilities were observed after the screening events. CONCLUSION The multiphasic, community-based approach proved feasible for both women and men and resulted in high rates of compliance with follow-up testing. This approach appears highly replicable it was conducted multiple times across the years with different screening targets, which could be further scaled elsewhere using the same technique.Silk fibers, which are protein-based biopolymers produced by spiders and silkworms, are fascinating biomaterials that have been extensively studied for numerous biomedical applications. Silk fibers often have remarkable physical and biological properties that typical synthetic materials do not exhibit. These attributes have prompted a wide variety of silk research, including genetic engineering, biotechnological synthesis, and bioinspired fiber spinning, to produce silk proteins on a large scale and to further enhance their properties. In this review, we describe the basic properties of spider silk and silkworm silk and the important production methods for silk proteins. We discuss recent advances in reinforced silk using silkworm transgenesis and functional additive diets with a focus on biomedical applications. We also explain that reinforced silk has an analogy with metamaterials such that user-designed atypical responses can be engineered beyond what naturally occurring materials offer. These insights into reinforced silk can guide better engineering of superior synthetic biomaterials and lead to discoveries of unexplored biological and medical applications of silk. Expected final online publication date for the Annual Review of Biomedical Engineering, Volume 22 is June 4, 2020. Please see http//www.annualreviews.org/page/journal/pubdates for revised estimates.Objective This study aimed to analyze the cognitive psychological process and to understand the decision-making process involved in university student passengers' seat belt usage behavior and the difference between front and rear seat belt use. Based on the Theory of Planned Behavior (TPB), two extended variables, namely, perceived accident severity and safety consciousness, were introduced. From the perspective of social psychology, we explored the psychological factors influencing university student passengers' seat belt usage behavior and the relationship between these factors.Methods The reliability and validity of 544 valid questionnaires were tested, and the differences were analyzed in the scores of the TPB variables between university student transportation majors and other majors. Finally, structural equation models were established to compare the factors affecting seat belt usage in the front and rear seats.Results The results show that the TPB could effectively explain and predict university student passengers' seat belt usage behavior. Significant differences were observed in the TPB variables, the extended variables and the behavioral intention scores between the university students majoring in transportation and those with other majors. In addition, significant differences were observed between front and rear seat belt usage. All psychological factors had significant positive effects on the seat belt usage behavior of the university student passengers, and all factors directly or indirectly affected seat belt usage behavior.Conclusions The present research adds further empirical evidence regarding the ability of the TPB to predict seat belt usage behavior. In addition, the current research results may provide a theoretical basis for policy-making aiming to improve university students' seat belt usage.