We here address the question whether the unique capacity of mesenchymal stem cells to re-establish tissue homeostasis depends on their potential to sense pathogen-associated molecular pattern and, in consequence, mount an adaptive response in the interest of tissue repair. After injection of MSCs primed with the bacterial wall component LPS into murine wounds, an unexpected acceleration of healing occurs, clearly exceeding that of non-primed MSCs. This correlates with a fundamental reprogramming of the transcriptome in LPS-treated MSCs as deduced from RNAseq analysis and its validation. A network of genes mediating the adaptive response through the Toll-like receptor 4 (TLR4) pathway responsible for neutrophil and macrophage recruitment and their activation profoundly contributes to enhanced wound healing. In fact, injection of LPS-primed MSCs silenced for TLR4 fails to accelerate wound healing. These unprecedented findings hold substantial promise to refine current MSC-based therapies for difficult-to-treat wounds. © 2020 The Authors. Published under the terms of the CC BY NC ND 4.0 license.Inspired by the flexibility of the bottom-up approach in terms of selecting molecular components and thus tailoring functionalities, a terpyridine derivative (1,2,4,5-tetrakis(4-(2,2'6',2″-terpyridyl)phenyl)benzene) (Tetra-tpy) is synthesized and coordinated with Co(II) ion to self-assemble into a nanosheet Co-sheet by a facile interface-assisted synthesis. The bis(terpyridine)-Co(II) complex nanosheet formed not only shows good stability, but also features the layered structure and rich electrochemical activity inherited from the embedded Co(terpyridine)2 motif. Thus, Co-sheet can serve as a cathode material for a dual-ion battery prototype, which exhibits a high utilization of redox-active sites, good cycling stability, and rate capability, thus expanding the potential application of this kind of easily prepared metal-complex nanosheets in the field of energy storage. © 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.Natural fluoride contamination of drinking water is a serious issue that affects several countries of the world. Its negative health impact is well documented in the East African Rift Valley, where water consumption with fluoride concentration greater than 1.5 mg/L can cause fluorosis to people. Within the framework of the EU Horizon2020 "FLOWERED" project we have first designed an effective defluoridation device based on innovative application of octacalcium phosphate (OCP), and then explored its acceptance within rural communities. The prototype (Flowered Defluoridator Device, FDD) essentially is composed by a 20 L tank and a recirculating pump that guarantees the interaction between water and OCP. The device is powered by a car battery for a fixed pumping working time using a fixed amount of OCP for every defluoridation cycle. The results of tests performed in the rural areas of Tanzania show that a standardized use of the prototype can lower the dissolved fluoride from an initial concentration of 21 mg/L to below the WHO drinkable limit of 1.5 mg/L in 2 hours without secondary negative effects on water quality. The approximate cost of this device is around 220 USD, while that of OCP is about 0.03 USD per liter of treated water. As any device, acceptance requires a behavioral change on behalf of rural communities that needed to be investigated. To this end, we piloted a survey to explore how psychological and socioeconomic factors influence the consumption of fluoride-free water. Results show that the adoption of FDD and OCP is more appealing to members of the rural communities who are willing to pay more and have a high consumption of water. Moreover, we suggest that given the low level of knowledge about fluorosis diseases, the government should introduce educational programs to make rural communities aware of the negative health consequences. https://www.selleckchem.com/products/gsk8612.html This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.BACKGROUND Androgenetic alopecia (AGA) is widely characterized as the main reason of hair loss which most of the time it has been reported in adults regarding dermatological consultation. Also, it is broadly believed that the progressive miniaturization of hair follicles could be usually different at the age of onset. AIMS Over the last decade, detecting and understanding newer genetic basis in AGA lead to provide better therapeutic approaches. We have highlighted on an evidence-based method to treat AGA whose incidence is increasing significantly in our country. PATIENTS/METHODS In this study, 9 adults with AGA including 5 women and 4 men, age range of 25-55 years, were treated with a combination of minoxidil (20%) by micro-needling and carboxytherapy mediated by needling. All cases had a normal physical development. Hair numbers (density) and diameter were calculated using trichograms before and after treatment. Hair growth was assessed by the pull test as well. RESULTS Our results showed that combination of high concentration of minoxidil and carboxygas extremely can increase the level of hair growth. CONCLUSION Our treatment effects on the terminal follicles using needling make sticky follicles become progressively smaller as a result of mechanical forces. © 2020 Wiley Periodicals, Inc.OBJECTIVE To characterize diagnostic findings, test-retest repeatability, and correlations among lacrimal tests in dogs of diverse cephalic conformations. ANIMAL STUDIED Fifty healthy dogs (25 brachycephalic, 25 nonbrachycephalic). PROCEDURES A series of diagnostics were performed in each dog, allowing for a 10-minute interval between tests and repeating each test 24 hours later under similar conditions corneal tactile sensation (CTS), strip meniscometry test (SMT), phenol red thread test (PRTT), endodontic absorbent paper point tear test (EAPPTT), Schirmer tear test-1 without (STT-1) or with nasolacrimal stimulation (NL-STT1), and Schirmer tear test-2 (STT-2). RESULTS Mean ± SD test values were lower in brachycephalic vs. nonbrachycephalic dogs (except for SMT; 7.4 ± 2.0 mm/5 seconds vs 7.3 ± 2.4 mm/5 seconds), with statistically significant differences noted for CTS (1.8 ± 0.5 cm vs 3.4 ± 0.8 cm), PRTT (37.2 ± 4.0 mm/15 seconds vs 41.1 ± 5.5 mm/15 seconds), STT-1 (20.1 ± 3.4 mm/min vs 23.3 ± 5.7 mm/min), STT-2 (13.