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To address this, we optimized the high-throughput, high-resolution fluorescent PCR-based fragment analysis method to develop MultiFRAGing - a robust and cost-effective method to genotype multiple targets in a single reaction. https://www.selleckchem.com/products/pki587.html Our approach can identify indels in up to four targets from a single reaction, which represents a four-fold increase in genotyping throughput. This method can be used by any laboratory with access to capillary electrophoresis-based sequencing equipment.The aim of the current study was to suggest a multi-hazard probability assessment in Fars Province, Shiraz City, and its four strategic watersheds. At first, we construct maps depicting the most effective factors on floods (12 factors), forest fires (10 factors), and landslides (10 factors), and used the Boruta algorithm to prioritize the impact of each respective factor on the occurrence of each hazard. Subsequently, flood, landslides, and forest fire susceptibility maps prepared using a Random Forest (RF) model in the R statistical software. Results indicate that 42.83% of the study area are not susceptible to any hazards, while 2.67% of the area is at risk of all three hazards. The results of the multi-hazard map in Shiraz City indicate that 25% of Shiraz city is very susceptible to flooding, while 16% is very susceptible to landslide occurrences. For four strategic watersheds, it is notable that in the Dorodzan Watershed, landslides and floods are the most important hazards; whereas, flood occurrences cover the largest area of the Maharlou Watershed. In contrast, the Tashk-Bakhtegan Watershed is so sensible to floods and landslides, respectively. Finally, in the Ghareaghaj Watershed, forest fire ranks as the strongest hazard, followed by floods. The validation results indicate an AUC of 0.834, 0.939, and 0.943 for the flood, landslide, and forest fire susceptibility maps, respectively. Also, other accuracy measures including, specificity, sensitivity, TSS, CCI, and Gini coefficient confirmed results of the AUC values. These results allow us to forecast the spatial behavior of such multi-hazard events, and researchers and stakeholders alike can apply them to evaluate hazards under various mitigation scenarios.An amendment to this paper has been published and can be accessed via a link at the top of the paper.In this study we have utilized an optical clearing method to allow visualization of a heretofore undescribed subpleural acinar structural organization in the mammalian lung. The clearing method enables visualization of the lung structure deep below the visceral pleura in intact inflated lungs. In addition to confirming previous observations that the immediate subpleural alveoli are uniform in appearance, we document for the first time that the subpleural lung parenchyma is much more uniformly organized than the internal parenchyma. Specifically, we report that below the surface layer of alveoli, there is a striking parallel arrangement of alveolar ducts that all run perpendicular to the visceral pleural surface. A three dimensional visualization of alveolar ducts allowed for a calculation of the average inner to outer duct diameter ratio of 0.53 in these subpleural ducts. This unique, self-organizing parallel duct structure likely impacts both elastic recoil and the transmission of tethering forces in healthy and diseased lungs.Memory abnormalities are considered a core feature of posttraumatic stress disorder (PTSD). Studies attempting to quantify such memory dysfunction in PTSD have reported that individuals with this disorder exhibit selective memory bias toward negative material. The low expression Met allele of brain-derived neurotrophic factor (BDNF) Val66Met polymorphism has been associated with the aetiology of PTSD and with memory abnormalities. It is therefore possible that the BDNF Val66Met polymorphism can moderate the relationship between PTSD and memory bias. Here we examined this association in 50 civilian women with PTSD and 70 non-trauma-exposed healthy control women. All subjects were genotyped for the BDNF Val66Met (rs6265) polymorphism. Negative memory bias was assessed using a recognition memory task. Patients showed significantly greater negative memory bias compared to controls. In patients, negative memory bias significantly increased with increasing numbers of Met alleles; while no significant relationship was seen in controls. Further pairwise analyses revealed that patients with the Met allele had significantly greater negative memory bias than controls. These results suggest that the relationship between PTSD and negative memory bias can be moderated by the BDNF Val66Met polymorphism. More studies are needed to further clarify the relationship between this polymorphism and memory abnormalities in PTSD.Fifty patients with unexplained fever and poor outcomes presented at Irrua Specialist Teaching Hospital (ISTH) in Edo State, Nigeria, an area endemic for Lassa fever, between September 2018 - January 2019. After ruling out Lassa fever, plasma samples from these epidemiologically-linked cases were sent to the African Centre of Excellence for Genomics of Infectious Diseases (ACEGID), Redeemer's University, Ede, Osun State, Nigeria, where we carried out metagenomic sequencing which implicated yellow fever virus (YFV) as the etiology of this outbreak. Twenty-nine of the 50 samples were confirmed positive for YFV by reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR), 14 of which resulted in genome assembly. Maximum likelihood phylogenetic analysis revealed that these YFV sequences formed a tightly clustered clade more closely related to sequences from Senegal than sequences from earlier Nigerian isolates, suggesting that the YFV clade responsible for this outbreak in Edo State does not descend directly from the Nigerian YFV outbreaks of the last century, but instead reflects a broader diversity and dynamics of YFV in West Africa.

Eventually, within the Ghareaghaj Watershed, forest fire ranks once the strongest threat, followed closely by floods. The validation results indicate an AUC of 0.834, 0.939, and 0.943 when it comes to flooding, landslide, and forest fire susceptibility maps, respectively. Additionally, various other precision steps including, specificity, sensitiveness, TSS, CCI, and Gini coefficient verified outcomes of the AUC values. These results let us predict the spatial behavior of such multi-hazard activities, and researchers and stakeholders alike can apply them to guage dangers under numerous mitigation scenarios.An amendment to this paper is posted and may be accessed via a hyperlink near the top of the paper.In this research we've used an optical clearing approach to enable visualization of a heretofore undescribed subpleural acinar structural organization within the mammalian lung. The clearing strategy enables visualization associated with lung framework deep below the visceral pleura in intact inflated lung area. Along with confirming past https://tgfbeta-receptor.com/index.php/a-review-of-your-ethnobotanical-benefit-phytochemistry-pharmacology-poisoning-along-with-qc/ observations that the instant subpleural alveoli are consistent in features, we document when it comes to first time that the subpleural lung parenchyma is more consistently arranged compared to the interior parenchyma. Particularly, we report that underneath the area level of alveoli, discover a striking parallel arrangement of alveolar ducts that all run perpendicular towards the visceral pleural surface. A three dimensional visualization of alveolar ducts allowed for a calculation for the normal inner to external duct diameter proportion of 0.53 in these subpleural ducts. This excellent, self-organizing parallel duct structure likely impacts both elastic recoil plus the transmission of tethering forces in healthy and diseased lungs.Memory abnormalities are considered a core function of posttraumatic anxiety disorder (PTSD). Studies trying to quantify such memory dysfunction in PTSD have reported that those with this disorder exhibit selective memory prejudice toward negative material. The reduced phrase Met allele of brain-derived neurotrophic aspect (BDNF) Val66Met polymorphism was associated with the aetiology of PTSD sufficient reason for memory abnormalities. It is therefore possible that the BDNF Val66Met polymorphism can moderate the relationship between PTSD and memory bias. Right here we examined this relationship in 50 civilian ladies with PTSD and 70 non-trauma-exposed healthy control females. All topics were genotyped for the BDNF Val66Met (rs6265) polymorphism. Bad memory prejudice had been considered making use of a recognition memory task. Clients revealed somewhat better negative memory bias in comparison to settings. In patients, unfavorable memory bias somewhat increased with more and more Met alleles; while no significant relationship was seen in settings. Further pairwise analyses disclosed that patients with the Met allele had notably greater unfavorable memory bias than controls. These results claim that the relationship between PTSD and negative memory prejudice may be moderated by the BDNF Val66Met polymorphism. Even more studies are needed to advance clarify the connection between this polymorphism and memory abnormalities in PTSD.Fifty clients with unexplained fever and bad outcomes presented at Irrua professional training Hospital (ISTH) in Edo State, Nigeria, a location endemic for Lassa temperature, between September 2018 - January 2019. After ruling on Lassa fever, plasma samples because of these epidemiologically-linked cases had been provided for the African Centre of Excellence for Genomics of Infectious Diseases (ACEGID), Redeemer's University, Ede, Osun State, Nigeria, where we carried out metagenomic sequencing which implicated yellow fever virus (YFV) due to the fact etiology of this outbreak. Twenty-nine of this 50 examples were verified positive for YFV by reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR), 14 of which resulted in genome system. Maximum probability phylogenetic analysis uncovered why these YFV sequences formed a tightly clustered clade much more closely pertaining to sequences from Senegal than sequences from earlier Nigerian isolates, suggesting that the YFV clade responsible for this outbreak in Edo State does not descend directly through the Nigerian YFV outbreaks associated with last century, but rather reflects a wider diversity and dynamics of YFV in western Africa. Right here we indicate the power of metagenomic sequencing for pinpointing continuous outbreaks and their particular etiologies and informing real-time public wellness responses, causing accurate and prompt infection management and control.Normalization processes for the qRT-PCR analysis of miRNA in biological examples tend to be suggested to reduce the variability due to pre-analytical aspects. Because there is no universal standardized normalization method for miRNA qRT-PCR studies, we carried out a throughout study to evaluate a panel of small non-coding RNAs (sncRNAs) as research gene candidate for biomarker researches in serum examples of patients with hepatocellular carcinoma (HCC). Five sncRNAs (miR-1280, miR-1275, SNORD-116, SNORD-68, and U6) had been plumped for as candidate of research genetics. This study included 122 clients with HCC and was arranged into a "pilot stage" consisting of 20 serum types of HCC patients, and a "validation phase" of 102 clients. Expression amount of these prospects were examined by qRT-PCR. Evaluation of gene security was carried out using four various integrative platforms (geNorm NormFinder, Bestkeeper, plus the Delta Ct strategy). To look for the gene security through the followup regarding the patient, we stretch the evaluation for the validation cohort at T1 (30 days after treatment) and T2 (6 thirty days after therapy). MiR-1280 had been identified as the most stably expressed research gene in both pilot and validation stage also throughout the followup.

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Video: 7 Step Bible Meditation Process Explored
Original blog with many hyperlinks, video and pictures, and great formatting is here:https://www.cwgministries.org/7-step-meditation-process-explored

Meditation: “God’s Spirit utilizing every faculty of my heart and mind, bringing forth revelation which ushers in transformation.”

Seven Steps in Biblical Meditation

Available as a free downloadable bookmark (shown at right).

Write: I copy the verse I am meditating on onto a piece of paper or 3X5 card, as instructed to in Deut. 17:18, and keep it with me to meditate on, memorize and mutter throughout the day(s). I also record this verse in my meditation/journal (which can be written, typed or verbally recorded).
Quiet Down: I become still in God’s presence, loving Him through soft soaking music (2 Kings 3:15,16) and/or praying in tongues (1 Cor. 14:14), or putting a smile on my face and picturing Jesus with me (Acts 2:25). I tune to His flowing thoughts, pictures and emotions (Jn. 7:37-39).
Reason: Come let us reason together (Isa. 1:18), meaning the Spirit guides my reasoning process. I ask, “Lord, what do You want to show me from this verse?” I tune to flow and revelation from the Holy Spirit bubbles up within me.
Speak & Imagine: I ponder the Scripture, personalizing and speaking it to myself softy over and over again until I can say it with my eyes closed. As I repeat the Scripture, I allow myself to see it with the eyes of my heart. I note what the picture is in my mind’s eye as I repeat the Scripture.
Feel God’s Heart: While seeing the above picture, I ask, “Lord, what does this Scripture reveal about Your heart toward me?” I feel His heart and journal it out.
Hear God’s Rhema: I put myself in the picture of this Scripture in my mind. I ask, “Lord, what are You speaking to me through this Scripture?” I tune to flowing thoughts and flowing pictures (God’s voice and vision) and I record this dialogue in my two-way journaling.
Act: I accept this revelation, repenting of any sin that is opposite of it and roaring at any obstacle that stands in the way of implementing it. I then speak it forth and act on it.

Our hearts burn within as He walks with us opening Scriptures to us (Lk. 24:32).

We are transformed as we look, and see what Jesus is doing (2 Cor. 3:18).

The Holy Spirit guides the above process, leading to more or less emphasis on any of the various steps, according to God’s wishes for the present moment and the personal needs one has. So we remain dependent upon Him throughout. For example, I may need more or less time to quiet myself in His presence or more or less time in Spirit-led “reasoning,” or more or less time in speaking it, or feeling God’s heart in it, or doing two-way journaling about it, or roaring at the enemy to get his lies out of my head and his hands off my being. So I allow the flow of the Holy Spirit to guide me through the steps of this meditation process.
A beautiful example of meditating upon Luke 2:25-38 (the story of Simeon and Anna in the temple).

Linda - What an amazing experience, Lord. I am still overwhelmed with what I felt. I saw Simeon holding Jesus as an infant, worshipping God for the fulfillment of a promise that God had given him. While he’s holding baby Jesus, his old friend Anna walks up and sees him holding Jesus, weeping and worshipping, and Anna knows immediately that this is what she had been praying and fasting for all her life. Anna goes and wraps her right arm around baby Jesus as Simeon is still holding him then her left hand is extended toward heaven as she worships too. I am a bystander but I walk over and ask permission to hold Jesus. Mary and Joseph nod and I sob as the most wonderful gift ever is placed in my arms. Thank You, Lord. I love You.

Jesus - You are welcome, beloved. I said the same thing when I held you in My arms when you were born anew!
How to Meditate on a Topic (Utilize the free software, e-Sword)

Be led by God to the topic: God will show you the topic He wants you to explore, by bringing it to your consciousness through thoughts, the comment of a friend or a book, or a presenting need in your life which demands the revelation and power of God to overcome (Jn. 16:13).
Be cleansed by His blood: Approach your meditation time by drawing near to the Lord, repenting of all sins and asking for and receiving the cleansing of His blood (Heb. 10:22).
Be humble and teachable: Ask for the Holy Spirit to reveal truth to you (Eph. 1:17,18). Be willing to discover and embrace His truth, no matter what it costs (reputation, pride, ego, job, financial security, etc.).
Be fearless: Some churches will excommunicate, fire or shun a person who disagrees with the church’s belief. Thus fear hinders many from pursuing truth. Put your whole trust in God to sustain you, even if you are shunned or rejected by organized religion.
Be whole-hearted in your search: Seek the Lord with your whole heart (i.e. presenting all your faculties to the Lord to fill and to use) and you will find Him (Jer. 29:13). Ask the Holy Spirit to guide and fill your heart and then tune to flowing thoughts, flowing pictures, flowing emotions.
Let the Holy Spirit guide you in the use of the following Bible tools:

A good concordance such as Strong’s Exhaustive Concordance and the King James Concordance (gives you every verse where a specific Hebrew or Greek word is used).
Some good Bible dictionaries such as Strong’s Hebrew and Greek Dictionaries, Brown – Driver – Briggs Dictionary, Vines Complete Bible Dictionary of New Testament Words, Vines Complete Dictionary of Old Testament Words.
Miscellaneous analytical tools such as Nave’s Topical Bible, and Manners and Customs of the Bible, etc.
Interpretive tools such as exegetical commentaries, expository commentaries and devotional commentaries.

Receive counsel: Wisdom and safety come from receiving counsel, input and confirmation from the 5-fold team God has given to you (Prov. 11:14; Eph. 4:11; 2 Cor. 13:1).

Most of the above tools (plus many more) are available electronically in the free software, e-Sword. Our online college course, BIB390 Biblical Research Methods from Christian Leadership University provides excellent training on how to research a topic using these tools.
An Example of the 7 Step Meditation Process

Meditation: “God’s Spirit utilizing every faculty of my heart and mind,
bringing forth revelation which ushers in transformation.”

Write: I copy the verse by hand onto a piece of paper or 3X5 card (Deut. 17:18) and keep it with me to meditate on, memorize and mutter throughout the day(s). I also record this verse in my meditation/journal (which can be written, typed or verbally recorded).

Isaac went out to meditate in the field toward evening (Gen. 24:63).

Quiet Down: I become still in God’s presence, loving Him through soft soaking music (2 Kings 3:15,16) and/or praying in tongues (1 Cor. 14:14), or putting a smile on my face and picturing Jesus with me (Acts 2:25). I tune to His flowing thoughts, pictures and emotions (Jn. 7:37-39).

Used the Sea of Galilee Quieting Exercise, picturing myself together with Jesus and tuned to flow.

Reason: I reason together with God (Isa. 1:18), meaning the Spirit guides my reasoning process (i.e. through flow). “Lord, what do You want to show me about any of the following: the context of the verse, the Hebrew/Greek definitions of the key words in the verse, any cultural understandings?” (“How to Meditate on a Topic” above details these steps.)

Meditation is a lifestyle that I have ordained. Do it in the evening. It is better than watching TV. As you do it, I can and will bring the choicest provisions of life to you. In this case, Isaac lifted up his eyes and saw the gift of his future wife who was being brought to him by his servant. His servant had discovered her in a distant land through a divine appointment. I also bring good gifts to you from distant places while you honor Me by making meditation your lifestyle. For when you honor Me by inviting Me into your everyday life, I honor you by bringing to you divine appointments. Honor Me with your lifestyle. Let your lifestyle be one of ongoing meditation.

Speak & Imagine: I ponder the Scripture speaking it to myself softy over and over again until I can say it with my eyes closed. As I repeat the Scripture, I allow myself to see it with the eyes of my heart. I note what the picture is in my mind’s eye as I repeat the Scripture.

I see Isaac walking in a field in the evening and pondering as he walks along. I see Jesus at his side, speaking with him.

Feel God’s Heart: While seeing the above picture, I ask, “Lord, what does this Scripture reveal about Your heart toward me?” I feel His heart and journal it out.

Mark, I love to walk with you in the cool of the day. This was My original design. This allows you to hear My thoughts and receive My wisdom, My counsel and My blessing. It is My desire to love you and care for you and provide for you and this is one key way I can do that if you allow Me to. Come experience My heart toward you in the cool of the day. Come meditate in the cool of the day.

Hear God’s Rhema: I put myself in the picture of this Scripture in my mind. I ask, “Lord, what are You speaking to me through this Scripture?” I tune to flowing thoughts and flowing pictures (God’s voice and vision) and I record this dialogue in my two-way journaling.

Mark, there is SO much I want to reveal to you and I do it as we take these walks together in the cool of the day. You see, this was My pattern in the Garden of Eden. I chose for it to be our pattern also, that we walk together down the road of life. Will you come to Me in the cool of the day and meditate in My presence, allowing Me to minister grace to you on a daily basis? You can ponder the specific needs and situations you are surrounded by and present them to Me and I will give you revelation and insight as to how to best handle them and respond to them. I will do this daily if you will walk with Me daily. You will experience ideas and understanding beyond your natural ability. You will accomplish beyond your natural giftings.

Act: I accept this revelation, repenting of any sin that is opposite of it and roaring at any obstacle that stands in the way of implementing it. I then speak it forth and act on it.

Lord, I accept this awesome invitation from You, my Lord and my Redeemer. What a gracious gift You have offered me, and all I have to do is say yes, and we walk together. I receive Your life and fullness into my heart and life. Lord, what an amazing offer You are making. I roar at every false belief that I can do it on my own. That is a lie from satan. I renounce humanism and rationalism. Get out of my life, now! I choose to die daily and come alive only to Jesus, who is my life.

Lord, I will walk with You and talk with You in the cool of the day and I will present to You the issues I am facing and ask for Your wisdom and insight on them. I thank You, Lord, for Your gracious wisdom, revelation and strength which flow so freely!!!

Our hearts burn within as He walks with us opening Scriptures to us (Lk. 24:32).
We are transformed as we look, and see what Jesus is doing (2 Cor. 3:18).
Let’s Begin Meditating and Enjoying Life-transforming Revelation & Empowerment

Select one verse a day to meditate on. (It could be that some days you want to continue to meditate on the verse from the previous day.) Copy the above “7 Step Meditation Process” into your word processor and insert into it the Scripture you are meditating on for the day along with the revelation God gives you.

You could begin with the verses on meditation listed below and then move on to verses or topics which interest you. If you want more verses on the topic of meditation, refer to the Scriptures in the file “Meditation – Hebrew and Greek Definitions” for an additional 40+ verses. For topical lists of verses to meditate on, use the “Bible search” feature, available as part of the free software, e-Sword, and search for words that deal with the topic(s) you want to explore in-depth.

Since revelation promotes transformation, I recommend meditating in areas where you are seeking change. If I desire to move into God’s miracle-working power, I would meditate on the words for power and healing, and compassion. If I need to discover the many ways He heals, I will meditate on all the verses in the Bible which speak of health, heal, healed, healing. If I need improved interpersonal relationships, I will meditate on the “one another” commands in the New Testament. Additional meditation topics and resources are suggested at the end of this file. (Note: you can explore my meditations on the above keywords by typing them in the search bar at www.cwgministries.org).

I will stay with a topic until the revelation is complete, the enemy has been roared at and is defeated, and I am a living testimony of God’s grace in that area. Then the Holy Spirit will move me on to the next area where I need revelation, transformation, and breakthrough. This is the process of life. This is the way we are called to live, so do not miss out on life! Since I am a teacher, I consolidate each year of God’s revelation and transformation to me into a training book in order to easily pass it on.
Blogs On Meditation and Using the Eyes of Your Heart as You Approach God


How to Meditate on Scripture & Receive Life-Transforming Revelation
Hebrew and Greek Definitions of Meditation
Does the Bible REALLY Say “Study to Show Yourself Approved”?
Western Study Versus Biblical Meditation
7 Step Meditation Process Explored
7 Step Meditation Bookmark (shown at right)
Healing Scriptures for Meditation
4 Keys to Hearing God’s Voice
What Does the Bible Teach about Visualization?
You Can See Visions! They Are a Pathway to God
Poised Before Almighty God
Lord, When Is “Priming The Pump” Acceptable and Unacceptable?
Initiative - Should I Take It? by Charity Kayembe
Help! I Have Trouble Seeing Vision!
Protestantism's 500-Year-Old Bitter Root Judgment and Inner Vow (a chance to repent)
Training Your Child to Encounter Jesus - Godly Imagination & Visions
How to Receive Revelation Knowledge

Books And Courses On Meditation And Spiritual Formation

Hearing God Through Biblical Meditation book
Meditation: How to Study the Bible in the Presence of God ebook
The Art of Biblical Meditation Interactive Online Course from School of the Spirit
Training Package - 4 Keys To Hearing God's Voice (One key is to use vision)
Training Package - Hear God Through Your Dreams (Dreams utilize imagery)
Book - Am I Being Deceived? (Difference between New Agers and Christians)
Book - Secret Place (Examples of God painting beautiful scenes during prayer times)
Divine Healing Toolbox CD/DVD Set (using vision while praying for healing)
Training Package - Prayers That Heal the Heart (Inner healing prayer utilizes vision)
Sound Doctrine Through Revelation Knowledge by Mark and Patti Virkler – an alternative to traditional hermeneutics.
The Great Mystery – Christ in You by Mark and Patti Virkler – Explores all New Testament verses that describe what Jesus provides for you through His indwelling Spirit.
How to Walk by the Spirit by Mark and Patti Virkler – Explores Scriptures concerning the heart
Intimacy with the Holy Spirit by Mark and Patti Virkler– Explores Scriptures concerning the Holy Spirit
How Do You Know? by Mark and Patti Virkler – Explores hundreds of verses which reveal God’s prescribed methodology for discovering truth.

© 2013 Mark & Patti Virkler – Original source can be found at www.cwgministries.org/meditation
Permission given to freely distribute in its entirety, provided all hyperlinks remain intact.